PROTEOMICS AND GENOMICS
BIO5PAG
2020
Credit points: 15
Subject outline
In this subject, students will gain theoretical experience with molecular biology, protein expression (in prokaryotes and eukaryotic systems) and protein chemistry techniques most commonly used in the early stages of proteomics and genomics research. The students will also learn a wide variety of advanced topics in proteomics and genomics including (but not limited to) the next generation cloning techniques such as 'Gibson Assembly', 'In-Fusion', 'SLICE', 'LIC' and 'TA' cloning, CRISPR-mediated gene editing, Next Gen Sequencing, RNAseq, epigenetics, post-translational modifications and mass spectrometry (including SILAC). In workshops, which are an integral part of this subject, students will learn about practical problem solving related to various subjects taught in this course. Students will also use online data analysis software to identify DNA and protein molecules and algorithms to identify CRISPR gene targets, RNAseq analysis and methylation analysis. In completing tasks for this subject, students will gain confidence in applying their knowledge to future research problems.
School: Molecular Sciences (Pre 2022)
Credit points: 15
Subject Co-ordinator: Hamsa Puthalakath
Available to Study Abroad/Exchange Students: Yes
Subject year level: Year Level 5 - Masters
Available as Elective: No
Learning Activities: N/A
Capstone subject: No
Subject particulars
Subject rules
Prerequisites: Must be enrolled in one of the following courses: SGBB, SMBB, SMBM, SMBT, SMNT, or SZHSMN All other students require coordinator approval
Co-requisites: N/A
Incompatible subjects: N/A
Equivalent subjects: N/A
Quota Management Strategy: N/A
Quota-conditions or rules: N/A
Special conditions: N/A
Minimum credit point requirement: N/A
Assumed knowledge: N/A
Career Ready
Career-focused: No
Work-based learning: No
Self sourced or Uni sourced: N/A
Entire subject or partial subject: N/A
Total hours/days required: N/A
Location of WBL activity (region): N/A
WBL addtional requirements: N/A
Graduate capabilities & intended learning outcomes
Graduate Capabilities
Intended Learning Outcomes
Melbourne (Bundoora), 2020, LTU Term 5, Blended
Overview
Online enrolment: Yes
Maximum enrolment size: N/A
Subject Instance Co-ordinator: Hamsa Puthalakath
Class requirements
LectureWeek: 37 - 42
Two 2.00 hours lecture per week on any day including weekend during the day from week 37 to week 42 and delivered via online.
Pre-recorded
WorkShopWeek: 37 - 42
Two 2.00 hours workshop per week on weekdays during the day from week 37 to week 42 and delivered via video conference.
Assessments
| Assessment element | Category | Contribution | Hurdle | % | ILO* |
|---|---|---|---|---|---|
1 x 1,000-word assignmentEach group will be allocated a research paper from a leading scientific journal. They will need to discuss the paper and prepare a multimedia eg. a voice over PowerPoint file, encompassing various aspects of the assessment as detailed in section ILO-02 learning activities. Each student in a group will be assessed individually based on their input into the various aspects of the presentation. | N/A | N/A | No | 15 | SILO2, SILO3, SILO4 |
2-hour written examination(2000 word equivalent)Students will have a set time frame (120 min) to complete a series of questions covering all aspects of the subject curriculum. Questions may be theoretical, technical or analytical in nature. | N/A | N/A | No | 40 | SILO3, SILO4 |
7 x problem based workshop scenario reports (5% each) (2,000-words equivalent total)Each workshop is based on the theme/ subject that was taught in the previous lecture. The students may be asked to critically assess and write a report on a technical video or may be given a proteomics/molecular genetics-based problem to be solved. Students are expected to use online, web-based tools to solve these problems including BLAST, BLAT (UCSC genome browser) and ExPASy (SIB Bioinformatics Resource) | N/A | N/A | No | 35 | SILO1, SILO3, SILO4 |
1 x multiple choice quiz on LMS(500-words equivalent)Students will be asked to complete a series of questions covering key concepts outlined in the lecture material or provide answers to a problem-solving activity. | N/A | N/A | No | 10 | SILO3, SILO4 |