PROTEIN CHEMISTRY
BIO5PRO
2014
Credit points: 15
Subject outline
In this unit students will gain hands on experiecne with common methods of protein purification and analysis. This will include protein extraction and solubilization, protein concentration determination, ion exchange, gel filtration and affinity chromatography, gel electrophoresis and immunoblotting. Students will apply these techniques to purify and analyze proteins from complex mixtures.
Faculty: Faculty of Science, Tech & Engineering
Credit points: 15
Subject Co-ordinator: Linda Ward
Available to Study Abroad Students: Yes
Subject year level: Year Level 5 - Masters
Exchange Students: Yes
Subject particulars
Subject rules
Prerequisites: N/A
Co-requisites: N/A
Incompatible subjects: N/A
Equivalent subjects: N/A
Special conditions: N/A
Learning resources
Readings
| Resource Type | Title | Resource Requirement | Author and Year | Publisher |
|---|---|---|---|---|
| Readings | Protein Methods | Recommended | Bollag D.M., Rozycki M.D. and Edelstein S.J. | SECOND EDITION, WILEY-LISS |
Melbourne, 2014, Semester 1, Day
Overview
Online enrolment: Yes
Maximum enrolment size: N/A
Enrolment information:
Subject Instance Co-ordinator: Linda Ward
Class requirements
PracticalWeek: 23 - 27
Three 6.0 hours practical per week on weekdays during the day from week 23 to week 27 and delivered via face-to-face.
"3x6 hour practicals in subsequent weeks"
WorkShopWeek: 23 - 22
Three 4.0 hours workshop per week on weekdays during the day from week 23 to week 22 and delivered via face-to-face.
"3x4hour workshops in first week"
Assessments
| Assessment element | Comments | % |
|---|---|---|
| class participation and performance | 10 | |
| one 2-hour practical test | 25 | |
| one 20-minute group oral presentation | 10 | |
| practical reports or worksheets (equivalent to 1,000 words) | 25 | |
| two scientific writing and communication exercises (equivalent to 1,500 words) | 30 |