Credit points: 15

Subject outline

In this unit students will gain hands on experiecne with common methods of protein purification and analysis. This will include protein extraction and solubilization, protein concentration determination, ion exchange, gel filtration and affinity chromatography, gel electrophoresis and immunoblotting. Students will apply these techniques to purify and analyze proteins from complex mixtures.

FacultyFaculty of Science, Tech & Engineering

Credit points15

Subject Co-ordinatorLinda Ward

Available to Study Abroad StudentsYes

Subject year levelYear Level 5 - Masters

Exchange StudentsYes

Subject particulars

Subject rules



Incompatible subjectsN/A

Equivalent subjectsN/A

Special conditionsN/A

Learning resources


Resource TypeTitleResource RequirementAuthor and YearPublisher
ReadingsProtein MethodsRecommendedBollag D.M., Rozycki M.D. and Edelstein S.J.SECOND EDITION, WILEY-LISS

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Start date between: and    Key dates

Melbourne, 2014, Semester 1, Day


Online enrolmentYes

Maximum enrolment sizeN/A

Enrolment information

Subject Instance Co-ordinatorLinda Ward

Class requirements

Practical Week: 23 - 27
Three 6.0 hours practical per week on weekdays during the day from week 23 to week 27 and delivered via face-to-face.
"3x6 hour practicals in subsequent weeks"

WorkShop Week: 23 - 22
Three 4.0 hours workshop per week on weekdays during the day from week 23 to week 22 and delivered via face-to-face.
"3x4hour workshops in first week"


Assessment elementComments%
class participation and performance10
one 2-hour practical test25
one 20-minute group oral presentation10
practical reports or worksheets (equivalent to 1,000 words)25
two scientific writing and communication exercises (equivalent to 1,500 words)30