PROTEIN CHEMISTRY

BIO5PRO

2015

Credit points: 15

Subject outline

This subject will provide students with the opportunity to enhance their practical laboratory skills through a variety of protein chemistry applications. Practical classes will require students to develop protocols and carry out laboratory techniques such as protein expression and purification, electrophoresis (SDS-PAGE), reporter assays and biochemical activity assays. In completing tasks for this subject, students are expected to develop skills in critical thinking, data analysis, teamwork and scientific communication.

SchoolSchool of Molecular Sciences/LIMS

Credit points15

Subject Co-ordinatorLinda Ward

Available to Study Abroad StudentsYes

Subject year levelYear Level 5 - Masters

Exchange StudentsYes

Subject particulars

Subject rules

PrerequisitesN/A

Co-requisitesN/A

Incompatible subjectsN/A

Equivalent subjectsN/A

Special conditionsN/A

Graduate capabilities & intended learning outcomes

01. Understand, interpret and critically evaluate experimental protocols

Activities:
Students will be allocated a scientific method that they must convert into a step-by-step protocol. During workshops, students will research and evaluate the technical limitations of their protocol. For assessment, students must produce a learning resource that includes a practical guide and an instructional presentation for carrying out the procedure.

02. Develop and convey an understanding of protein chemistry techniques

Activities:
Students will complete worksheets that contain a combination of questions about key concepts covered in lectures, workshops and practical classes. Students will have the opportunity to discuss these problems with demonstrators and fellow students. These must be submitted for assessment and feedback

03. Prepare, perform, interpret and explain an experiment

Activities:
Students will use the protocols compiled by their group to carry out their laboratory work. Both the experimental data and results will be reported through a group oral presentation. All group members will be asked questions about their protocols and results. Students are expected to accurately explain the purpose of their experiments and the outcomes observed.

04. Practice gathering and reporting information from published literature and scientific resources

Activities:
Students will produce a product information sheet that has background information for the protein they produce in the laboratory. Workshop activities will focus on collating information from a variety of scientific resources and documenting this information in a concise manner.

05. Demonstrate effective collaboration and organisational skills through contributing to group work

Activities:
At the beginning of this unit, students will be assigned to project groups. Students will be required to work collaboratively to compile a set of protocols and to carry out their laboratory work. Each student is responsible for purifying and supplying a different component needed for the group's assay. The success of the group work will be measured during the presentation of the group's data.

06. Compile and present experimental data in a format appropriate for publication

Activities:
Workshop activities will provide students with the opportunity to develop skills in compiling and presenting their data for inclusion in their product information sheet. The product information sheet will resemble that of a commercially available protein and will be professionally researched, written and formatted.

Subject options

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Start date between: and    Key dates

Melbourne, 2015, Semester 1, Day

Overview

Online enrolmentYes

Maximum enrolment sizeN/A

Enrolment information

Subject Instance Co-ordinatorLinda Ward

Class requirements

Lecture Week: 10 - 22
One 1.0 hours lecture per week on weekdays during the day from week 10 to week 22 and delivered via face-to-face.

WorkShop Week: 10 - 15
One 2.0 hours workshop per week on weekdays during the day from week 10 to week 15 and delivered via face-to-face.

Practical Week: 16 - 22
One 4.0 hours practical per week on weekdays during the day from week 16 to week 22 and delivered via face-to-face.

Assessments

Assessment elementComments% ILO*
Class preparation, participation and performance10 02, 03, 05
Written practical exam30 01, 02
Group oral presentation10 02, 03, 05
Question sheets20 02, 03
Development and delivery of laboratory protocols20 01, 02, 05
Product information sheet10 03, 04, 06