Understanding dual nucleic acid specificities of SFPQ in reversible gene regulation

SFPQ has been reported to repress the transcription of several genes via direct binding to the promoter regions. Despite the presence of two RNA recognition motifs, the cognate RNA sequences of SFPQ are yet to be identified. However, several long noncoding RNAs (lncRNAs) that interact with SFPQ have been identified in the recent reports on the reversible regulatory roles of SFPQ. In the proposed mechanism of reversible gene regulation, the transcription repression exerted by SFPQ via direct binding to the promoter region can be reversed by its interactions with lncRNAs. This project aims to solve the structure of SFPQ in complex with nucleic acids to provide the structural basis of the reversible gene regulation.