Greening - Extracellular vesicles, exosomes, cancer biology and uterine biology
Dr David Greening
Bruce Stone Fellow in Biological Chemistry, College of Science, Health and Engineering
Within the past decade, extracellular vesicles (EVs) have emerged as critical mediators of intercellular communication, particularly involved in the transmission of biological signals and select cargo between cells, thereby regulating various pleiotropic biological processes. EVs exert diverse physiological and pathophysiological functions by horizontal transfer of protein, DNA, and RNA species between cells. There is now a growing awareness that predominant EV subtypes; exosomes from endosomal origin, and shed microvesicles from plasma membrane budding, can be further stratified into distinct subtypes. We investigate the mechanisms of distinct EV biogenesis and release, defining select EV classes (and subpopulations), which will be crucial for development of EV-based functions and clinical applications. The advent of quantitative mass spectrometry-based proteomics, in conjunction with advances in chemical labelling, cross-linking, molecular cell biology, and EV purification strategies, has contributed significantly to our improved characterization and understanding of the molecular composition and functionality of these distinct EV subpopulations.