Next-Generation Sequencing

Applications

• Small to medium scale sequencing capacity (<8 Gb)
• De novo genome sequencing of small to medium sized genomes
• Exome sequencing
• Transcriptome sequencing
• Genotyping by sequencing (RADseq)Targeted re-sequencing (e.g. sequencing of disease-associated genes in a patient cohort).

Capabilities

Regular

• 300 bp PE
• 25 million reads per run.

Capabilities

High-output

• 150 PE
• 400 million reads per run.

Mid-output

• 150 PE
• 130 million reads per run.

Applications

• De novo genome sequencing (unknown genomes)
• Targeted resequencing - multiplexed PCR amplicons > exomes > known genomes
• Transcriptome (RNA) sequencing (identify and quantify global mRNA expression patterns
• Global analysis of DNA-protein interactions using ChIP-seq
• Identification and quantification of micro RNAs without prior genome annotation
• Genotyping by sequencing (RADseq)
• Epigenetic sequencing of:
  • bisulfite-converted DNA
  • methylation-sensitive restriction digest-enriched fragments
  • anti-methyl C-precipitated fragments
  • chromatin IP of methyltransferases containing aza-labelled DNA.

Capabilities

High-output

• 125 bp PE
• 250 million reads per lane.

Rapid run

• 150 bp PE
• 150 million reads per lane.

Applications

• RNA, DNA and protein sample QC

Diagenode’s Bioruptor ® Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems.

User Manual