The single-cell omics platform is transforming our understanding of cardiac disease at the cellular level.
Managed by Associate Professor Alex Pinto, the platform features fluorescence activated cell sorting facilities, a 10X Genomics Chromium Controller, an Agilent Bioanalyser and high-performance computer systems.
“The cell sorting equipment and the 10X Genomics Chromium Controller allows us to isolate individual cells from complex mixtures,” Pinto explains. “The Agilent Bioanalyzer enables the quantity and quality of minute amounts of DNA from cells to be determined before sequencing.”
The technology enables researchers to understand complex tissues at the single-cell level in the context of development and disease.
“We can ensure high-quality samples and data are prepared to accurately study the genetics of individual cells,” says Pinto. “We examine a range of cells to determine their gene activation and their role in the development of disease. We can examine heart cells, for example, to understand what’s happening during heart failure, and identify new cellular and molecular targets for therapeutics.”
Using the single-cell omics platform, Pinto and his team developed the Cardiovascular Atlas (CLARA), which enables researchers to explore the cellular landscapes of cardiovascular and other tissues that influence cardiovascular health.
The platform also coordinates with other facilities in the Baker and Alfred Research Precinct including the preclinical cardiology platform, the microsurgery and imaging platform, and the proteomics research platform.
“The single-cell omics platform optimises every aspect of research, from study design and budgeting, to data analysis and publication,” explains Pinto. “It also supports grant preparation and researcher training initiatives.”
“But, most importantly, the platform helps researchers to take our understanding of cardiovascular disease to the next level. These discoveries ultimately inform prevention and treatment strategies, to enhance the health and wellbeing of our communities.”