Professor Michael Ryan
Head of Department of Biochemistry, Professor
Faculty of Science, Technology and Engineering
School of Molecular Sciences
Department of Biochemistry
La Trobe Institute for Molecular Science
LIMS1 Room 525, Melbourne (Bundoora)
BSc Hons, PhD La Trobe
Area of study
Biochemistry and Molecular Biology
After completing his PhD, Mike received an Alexander von Humboldt Fellowship and worked on mitochondrial protein import in the laboratory of Klaus Pfanner in Freiburg, Germany. In 2000, Mike joined La Trobe where he undertakes research into understanding the assembly of membrane protein complexes in mitochondria and defects in disease which cause energy generation defects. He also studies the machinery that controls mitochondrial dynamics. His research has been funded by grants from numerous bodies including the Australian Research Council, National Health and Medical Research Council, and the ARC Centre of Excellence for Coherent X-ray Science where he also leads the Biology program. In 2006, Mike received the Roche Medal from the ASBMB. In 2010 Mike was appointed to professor and later Head of Department. Mike also serves on the La Trobe Institute for Molecular Science Advisory Board and Executive.
Mitochondria biogenesis and disease
- Assembly of membrane protein complexes in health and disease
Mike lectures into Biochemistry and Molecular Biology at second and third year levels.
Gene Knockout Using Transcription Activator-like Effector Nucleases (TALENs) Reveals That Human NDUFA9 Protein Is Essential for Stabilizing the Junction between Membrane and Matrix Arms of Complex I. Stroud DA, Formosa LE, Wijeyeratne XW, Nguyen TN, Ryan MT. J Biol Chem. 2013 18;288(3):1685-90.
Mitochondrial dysfunction in a novel form of autosomal recessive ataxia. Murad NA, Cullen JK, McKenzie M, Ryan MT, Thorburn D, Gueven N, Kobayashi J, Birrell G, Yang J, Dörk T, Becherel O, Grattan-Smith P, Lavin MF. Mitochondrion. 2012 Nov 22. doi:pii: S1567-7249(12)00254-1.
Impaired folding of the mitochondrial small TIM chaperones induces clearance by the i-AAA protease. Baker MJ, Mooga VP, Guiard B, Langer T, Ryan MT, Stojanovski D. J Mol Biol. 2012 Dec 14;424(5):227-39.
Mitofusins 'bridge' the gap between oxidative stress and mitochondrial hyperfusion. Ryan MT, Stojanovski D. EMBO Rep. 2012 Oct;13(10):870-1.
Recent advances into the understanding of mitochondrial fission. Elgass K, Pakay J, Ryan MT, Palmer CS. Biochim Biophys Acta. 2013 Jan;1833(1):150-61.
Proteomic and Metabolomic Analyses of Mitochondrial Complex I-deficient Mouse Model Generated by Spontaneous B2 Short Interspersed Nuclear Element (SINE) Insertion into NADH Dehydrogenase (Ubiquinone) Fe-S Protein 4 (Ndufs4) Gene. Leong DW, Komen JC, Hewitt CA, Arnaud E, McKenzie M, Phipson B, Bahlo M, Laskowski A, Kinkel SA, Davey GM, Heath WR, Voss AK, Zahedi RP, Pitt JJ, Chrast R, Sickmann A, Ryan MT, Smyth GK, Thorburn DR, Scott HS. (2012) J Biol Chem. 287, 20652-63.
Tissue-specific splicing of an Ndufs6 gene-trap insertion generates a mitochondrial complex I deficiency-specific cardiomyopathy. Ke BX, Pepe S, Grubb DR, Komen JC, Laskowski A, Rodda FA, Hardman BM, Pitt JJ,Ryan MT, Lazarou M, Koleff J, Cheung MM, Smolich JJ, Thorburn DR. (2012) Proc Natl Acad Sci U S A. 109, 6165-70.
Next Generation Sequencing in molecular diagnosis: NUBPL mutations highlight the challenges of variant detection and interpretation. Tucker EJ, Mimaki M, Compton AG, McKenzie M, Ryan MT, Thorburn DR. 2012 Human Mutat. Feb;33(2):411-8.
Understanding mitochondrial complex I assembly in health and disease. Mimaki M, Wang X, McKenzie M, Thorburn DR, Ryan MT. Biochim Biophys Acta. 2011 Mar;1808(3):1002-11.
Mutations in the Gene Encoding C8orf38 Block Complex I Assembly by Inhibiting Production of the Mitochondria-Encoded Subunit ND1. McKenzie M, Tucker EJ, Compton AG, Lazarou M, George C, Thorburn DR, Ryan MT. 2011 J Mol Biol. 2;414(3):413-26.
Mutations in MTFMT underlie a human disorder of formylation causing impaired mitochondrial translation. Tucker EJ, Hershman SG, Köhrer C, Belcher-Timme CA, Patel J, Goldberger OA, Christodoulou J, Silberstein JM, McKenzie M, Ryan MT, Compton AG, Jaffe JD, Carr SA, Calvo SE, RajBhandary UL, Thorburn DR, Mootha VK. Cell Metab. 2011 Sep 7;14(3):428-34.
The regulation of mitochondrial morphology: intricate mechanisms and dynamic machinery. Palmer CS, Osellame LD, Stojanovski D, Ryan MT. Cell Signal. 2011 Oct;23(10):1534-45. Review.
MiD49 and MiD51, new components of the mitochondrial fission machinery. Palmer CS, Osellame LD, Laine D, Koutsopoulos OS, Frazier AE, Ryan MT. EMBO Rep. 2011 Jun;12(6):565-73.
Inhibition of Bak activation by VDAC2 is dependent on the Bak transmembrane anchor. Lazarou M, Stojanovski D, Frazier AE, Kotevski A, Dewson G, Craigen WJ, Kluck RM, Vaux DL, Ryan MT. J Biol Chem. 2010 Nov 19;285(47):36876-83.
Mitochondrial protein import machineries and lipids: A functional connection. Gebert N, Ryan MT, Pfanner N, Wiedemann N, Stojanovski D. Biochim Biophys Acta. 2010 Aug 7.
Mutation of the mitochondrial tyrosyl-tRNA synthetase gene, YARS2, causes myopathy, lactic acidosis, and sideroblastic anemia--MLASA syndrome. Riley LG, Cooper S, Hickey P, Rudinger-Thirion J, McKenzie M, Compton A, Lim SC, Thorburn D, Ryan MT, Giegé R, Bahlo M, Christodoulou J. Am J Hum Genet. 2010 Jul 9;87(1):52-9.
Assembly factors of human mitochondrial complex I and their defects in disease. Mckenzie M, Ryan MT. IUBMB Life. 2010 Jul;62(7):497-502.
Human Miltons associate with mitochondria and induce microtubule-dependent remodeling of mitochondrial networks. Koutsopoulos OS, Laine D, Osellame L, Chudakov DM, Parton RG, Frazier AE, Ryan MT. Biochim Biophys Acta. 2010 May;1803(5):564-74. Epub 2010 Mar 15.
Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndrome. Gebert N, Joshi AS, Kutik S, Becker T, McKenzie M, Guan XL, Mooga VP, Stroud DA, Kulkarni G, Wenk MR, Rehling P, Meisinger C, Ryan MT, Wiedemann N, Greenberg ML, Pfanner N. Curr Biol. 2009 Dec 29;19(24):2133-9
Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria. Lazarou M, Smith SM, Thorburn DR, Ryan MT, McKenzie M. FEBS J. 2009 276(22): 6427 - 6824
Chapter 18 Analysis of respiratory chain complex assembly with radiolabeled nuclear and mitochondrial encoded subunits. McKenzie M, Lazarou M, Ryan MT. Methods Enzymol. 2009. 456:321-39.
Assembly of mitochondrial complex I and defects in disease. Lazarou M, Thorburn DR, Ryan MT, McKenzie M. Biochim Biophys Acta. 2009 1793(1):78-88.
Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import. Baker MJ, Webb CT, Stroud DA, Palmer CS, Frazier AE, Guiard B, Chacinska A, Gulbis JM, Ryan MT. Mol. Biol. Cell. 2009 20(3):769-79. Epub 2008 Nov 26.
Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import. Mutation of C20orf7 disrupts complex I assembly and causes lethal neonatal mitochondrial disease. Sugiana C, Pagliarini DJ, McKenzie M, Kirby DM, Salemi R, Abu-Amero KK, Dahl HH, Hutchison WM, Vascotto KA, Smith SM, Newbold RF, Christodoulou J, Calvo S, Mootha VK, Ryan MT, Thorburn DR. Am J Hum Genet. 2008 83(4):468-78.
Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase. Stojanovski D, Milenkovic D, Müller JM, Gabriel K, Schulze-Specking A, Baker MJ, Ryan MT, Guiard B, Pfanner N, Chacinska A. J Cell Biol. 2008 183(2):195-202.
Mitochondrial protein-import machinery: correlating structure with function. Baker MJ, Frazier AE, Gulbis JM, Ryan MT. Trends Cell Biol. 2007 17(9):456-64. Review.
Human CIA30 is involved in the early assembly of mitochondrial complex I and mutations in its gene cause disease. Dunning CJ, McKenzie M, Sugiana C, Lazarou M, Silke J, Connelly A, Fletcher JM, Kirby DM, Thorburn DR, Ryan MT. EMBO J. 2007 26(13):3227-37.
Analysis of the assembly profiles for mitochondrial- and nuclear-DNA-encoded subunits into complex I. Lazarou M, McKenzie M, Ohtake A, Thorburn DR, Ryan MT. Mol Cell Biol. 2007 27(12):4228-37.
Analysis of mitochondrial subunit assembly into respiratory chain complexes using Blue Native polyacrylamide gel electrophoresis. McKenzie M, Lazarou M, Thorburn DR, Ryan MT. Anal Biochem. 2007 364(2):128-37.