PROTEOMICS AND GENOMICS

BIO5PAG

2020

Credit points: 15

Subject outline

In this subject, students will gain theoretical experience with molecular biology, protein expression (in prokaryotes and eukaryotic systems) and protein chemistry techniques most commonly used in the early stages of proteomics and genomics research. The students will also learn a wide variety of advanced topics in proteomics and genomics including (but not limited to) the next generation cloning techniques such as 'Gibson Assembly', 'In-Fusion', 'SLICE', 'LIC' and 'TA' cloning, CRISPR-mediated gene editing, Next Gen Sequencing, RNAseq, epigenetics, post-translational modifications and mass spectrometry (including SILAC). In workshops, which are an integral part of this subject, students will learn about practical problem solving related to various subjects taught in this course. Students will also use online data analysis software to identify DNA and protein molecules and algorithms to identify CRISPR gene targets, RNAseq analysis and methylation analysis. In completing tasks for this subject, students will gain confidence in applying their knowledge to future research problems.

SchoolMolecular Sciences

Credit points15

Subject Co-ordinatorHamsa Puthalakath

Available to Study Abroad/Exchange StudentsYes

Subject year levelYear Level 5 - Masters

Available as ElectiveNo

Learning ActivitiesN/A

Capstone subjectNo

Subject particulars

Subject rules

Prerequisites Must be enrolled in one of the following courses: SGBB, SMBB, SMBM, SMBT, SMNT, or SZHSMN All other students require coordinator approval

Co-requisitesN/A

Incompatible subjectsN/A

Equivalent subjectsN/A

Quota Management StrategyN/A

Quota-conditions or rulesN/A

Special conditionsN/A

Minimum credit point requirementN/A

Assumed knowledgeN/A

Career Ready

Career-focusedNo

Work-based learningNo

Self sourced or Uni sourcedN/A

Entire subject or partial subjectN/A

Total hours/days requiredN/A

Location of WBL activity (region)N/A

WBL addtional requirementsN/A

Graduate capabilities & intended learning outcomes

Graduate Capabilities

Intended Learning Outcomes

01. Apply appropriate bioinformatic software to analyse and critically interpret genetic or proteomic data
02. Investigate, critically analyse, synthesise and convey complex information in a disciplinary context.
03. Demonstrate knowledge and understanding of advanced theoretical concepts in molecular biology and protein chemistry
04. Interpret and critically evaluate experimental data

Subject options

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Start date between: and    Key dates

Melbourne (Bundoora), 2020, LTU Term 5, Blended

Overview

Online enrolmentYes

Maximum enrolment sizeN/A

Subject Instance Co-ordinatorHamsa Puthalakath

Class requirements

Lecture Week: 37 - 42
Two 2.00 h lecture per week on any day including weekend during the day from week 37 to week 42 and delivered via online.
"Pre-recorded"

WorkShop Week: 37 - 42
Two 2.00 h workshop per week on weekdays during the day from week 37 to week 42 and delivered via video conference.

Assessments

Assessment elementCommentsCategoryContributionHurdle% ILO*
1 x 1,000-word assignment Each group will be allocated a research paper from a leading scientific journal. They will need to discuss the paper and prepare a multimedia eg. a voice over PowerPoint file, encompassing various aspects of the assessment as detailed in section ILO-02 learning activities. Each student in a group will be assessed individually based on their input into the various aspects of the presentation.N/AN/AN/ANo15 SILO2, SILO3, SILO4
2-hour written examination (2000 word equivalent) Students will have a set time frame (120 min) to complete a series of questions covering all aspects of the subject curriculum. Questions may be theoretical, technical or analytical in nature.N/AN/AN/ANo40 SILO3, SILO4
7 x problem based workshop scenario reports (5% each) (2,000-words equivalent total) Each workshop is based on the theme/ subject that was taught in the previous lecture. The students may be asked to critically assess and write a report on a technical video or may be given a proteomics/molecular genetics-based problem to be solved. Students are expected to use online, web-based tools to solve these problems including BLAST, BLAT (UCSC genome browser) and ExPASy (SIB Bioinformatics Resource)N/AN/AN/ANo35 SILO1, SILO3, SILO4
1 x multiple choice quiz on LMS (500-words equivalent) Students will be asked to complete a series of questions covering key concepts outlined in the lecture material or provide answers to a problem-solving activity.N/AN/AN/ANo10 SILO3, SILO4