Zbtb11 deficiency and in vivo tracking of HSCs
We have shown that in a zebrafish model of Zbtb11 deficiency HSCs disappear not long after they are first made. This project aims to determine precisely when HSCs are lost and why they disappear. Live imaging of optically transparent zebrafish larvae by confocal microscopy allows fluorescently labelled HSCs to be tracked from when they first appear to when they disappear. This enables us to directly observe and quantify crucial aspects of early HSC development as they happen in real time.