The achievement was reached in April 2014, 15 years after the campus held its first graduation for 14 students in 1999.
Mildura 1000 graduate milestone
Vice-Chancellor Professor John Dewar said it was proud moment for La Trobe University, regional Victoria's largest University educator.
'Congratulations to today's graduates and the many more that have come before them,' Professor Dewar said.
'A University education can change people's lives and their career prospects. We are very proud to be a part of the Mildura community and to be able to make a difference.'
Professor Dewar said La Trobe was committed to a long-term strategy to reduce the regional brain drain of young Victorians to the capital cities.
'We know that one of the best ways to keep young people living and working in regional areas is to empower them with a tertiary education that allows them to graduate and get a job within that community or another regional community,' Professor Dewar said.
'Our presence in Mildura means students are able to study here while maintaining connections with family and friends.
'The results speak for themselves. More than two thirds of La Trobe's Mildura graduates have been employed in rural Victoria.'
Head of the Mildura Campus Dr Debbie Neal said having a University campus in Mildura has encouraged people to stay, study and work in the district.
'La Trobe University graduates have an impact on almost every organisation and aspect of life in the Sunraysia community,' Dr Neal said.
'There are teachers, nurses, para-health professionals, social workers, artists, graphic designers, business managers and accountants who have all studied at La Trobe.
You will find them in the largest organisations of this community and the smallest.
'Our graduates work and influence the directions of Mallee Family Care, Mildura Rural City Council, Mildura Private and Public Hospitals, accountancy firms and every state and private primary and secondary school in the district,' she said.
In 2010, 44 per cent of new students were the first in their family to attend university. Now almost two thirds of students are first in their family to attend university.
'La Trobe is creating real opportunities right here in Mildura and will continue to do so,' said Dr Neal.
Rebecca Wells, Communications and Liason Officer, Mildura Operations
T: +61 3 5051 4020 E: firstname.lastname@example.org