How do highly conserved regions of PrP control prion formation?

Much remains to be understood about how the normal cellular isoform of the prion protein undergoes structural changes to become the disease associated form. We have developed several approaches to study this aspect of prion biology using cellular, molecular and biophysical techniques. We have refined a model based around a highly conserved region of the prion protein. This project will uses a combination of structural and cell biological approaches to investigate the mechanism of prion inhibition through this conserved region of the prion protein.