MOLECULAR GENETICS AND GENOMICS LABORATORY COURSE

GEN3LAB

2018

Credit points: 15

Subject outline

Genetics and genome science hold the keys to understanding life on earth, Human development, health and disease at the molecular level. This subject introduces students to advanced methods in experimental molecular genetics. The topics covered include the molecular regulation of embryogenesis, Human clinical genetics, bioinformatics, Human variation, forensic science, cancer genetics, genomics applications and ethical implications. Students will receive advanced training in techniques essential for employment in biotechnology, medical and research laboratories, including safe laboratory practice, light microscopy, micro-pipetting, handling dangerous chemicals, laboratory record keeping and scientific report writing, 

SchoolSchool of Molecular Sciences/LIMS

Credit points15

Subject Co-ordinatorAdam Hart

Available to Study Abroad StudentsYes

Subject year levelYear Level 3 - UG

Exchange StudentsYes

Subject particulars

Subject rules

Prerequisites GEN2MHG or GEN3HMG

Co-requisites GEN3MGG

Incompatible subjectsN/A

Equivalent subjectsN/A

Special conditionsN/A

Readings

Resource TypeTitleResource RequirementAuthor and YearPublisher
ReadingsGenetics Analysis and PrinciplesRecommendedBrooker, RMCGRAW HILL 2011
ReadingsHuman Embryology and developmentalRecommendedCarlson, BELSEVIER 2013
ReadingsHuman Evolutionary GeneticsRecommendedJobling M.A, Hurles. M and Tyler-Smith CGARLAND SCIENCE 2004
ReadingsHuman Molecular Genetics 4th EditionRecommendedStrachan, T and Read, A.P.GARLAND SCIENCE 2011
ReadingsPrinciple of DevelopmentRecommendedWolpert, L and Tickle COXFORD 2010
ReadingsJaneway's Immunobiology 7th editionRecommendedMurphy, Travers and WalportGarland Science
ReadingsConcepts of Genetics, 10th, international editionRecommendedKlug et. al.,Pearson

Graduate capabilities & intended learning outcomes

01. Apply advanced molecular genetics laboratory methods to generate and analyse high quality experimental data.

Activities:
Students will learn and apply techniques for safe laboratory practice, laboratory record keeping, microscopy, micropippetting, electrophoresis, karyotyping, histochemistry, genomic DNA extraction, polymerase chain reaction, restriction enzyme digestion, image analysis and basic statistical analysis. Students will learn and apply best practice approaches for preparing genetics data for publication.
Related graduate capabilities and elements:
Literacies and Communication Skills
Inquiry and Analytical Skills
Personal and Professional Skills
Discipline -Specific Knowledge and Skills

02. Analyse and evaluate experimental and published data relating to molecular genetics experiments to prepare a comprehensive laboratory report.

Activities:
Information about the research area, experimental and data analysis techniques, and report preparation guidelines are outlined in the practical manual and associated online (moodle) resources and are further explained in practical class/es and tutorial/s. Students have to conduct experiments in the practical classes, analyse and write up experimental results.
Related graduate capabilities and elements:
Literacies and Communication Skills
Literacies and Communication Skills
Inquiry and Analytical Skills
Inquiry and Analytical Skills
Inquiry and Analytical Skills
Personal and Professional Skills
Personal and Professional Skills
Discipline -Specific Knowledge and Skills

03. Use bioinformatics to solve genetics problems.

Activities:
Information to complete assignments is available in the practical manual and online guidelines. Individually and in small groups, students will access online worksheets, tutorials, genome databases and bioinformatics tools to solve genetics problems: - Students have to complete bioinformatics worksheets using online tutorials and database mining tools. - Students generate VNTR and RFLP (DNA variation) data in the laboratory and then use online sequence analysis and comparison tools to discover ApoB and PTC genotype/phenotype corelation, then analyse and describe the evolution of these genotypes and traits. - Students use online databases and apply gene search and analysis tools to analyse clinical case study data.
Related graduate capabilities and elements:
Literacies and Communication Skills
Inquiry and Analytical Skills
Inquiry and Analytical Skills
Inquiry and Analytical Skills
Personal and Professional Skills
Personal and Professional Skills
Discipline -Specific Knowledge and Skills

04. Analyse, discuss and present published original research journal article findings to peers and a scientific audience.

Activities:
Journal Club presentations: Information necessary for completing this assignment is available online as well as in workshop guidelines. Students need to select, read and discuss a research article from a provided list. Students present a discussion and critique of the research study, followed by answering questions from the academics. Written feedback is given by academics, demonstrators and peers.
Related graduate capabilities and elements:
Literacies and Communication Skills
Literacies and Communication Skills
Literacies and Communication Skills
Inquiry and Analytical Skills
Inquiry and Analytical Skills
Inquiry and Analytical Skills
Personal and Professional Skills
Personal and Professional Skills
Personal and Professional Skills
Discipline -Specific Knowledge and Skills

05. Discuss and debate the ethical implications of Genetics research.

Activities:
In the first session, groups of 8 students will be provided with published opinion and research on current genetics topics such as: criminal DNA databases; control of DNA information; saviour siblings; paternity; predictive genetic testing; forensic medicine and Informed consent. Students will discuss the topic and undertake further independent research. In a second session students will form groups of four to take opposing sides in the discussion mediated by a tutor. Opposing groups will summarize their arguments in front of academics and class in the final session.
Related graduate capabilities and elements:
Literacies and Communication Skills
Inquiry and Analytical Skills
Inquiry and Analytical Skills
Personal and Professional Skills
Personal and Professional Skills
Personal and Professional Skills
Discipline -Specific Knowledge and Skills

Subject options

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Start date between: and    Key dates

Melbourne, 2018, Semester 1, Day

Overview

Online enrolmentYes

Maximum enrolment sizeN/A

Enrolment information

Subject Instance Co-ordinatorAdam Hart

Class requirements

Laboratory Class Week: 10 - 22
One 6.0 hours laboratory class every two weeks on weekdays during the day from week 10 to week 22 and delivered via face-to-face.

WorkShop Week: 10 - 22
One 3.0 hours workshop every two weeks on weekdays during the day from week 10 to week 22 and delivered via face-to-face.

Assessments

Assessment elementComments% ILO*
Weekly Pre-lab online activity and quiz (900 words equivalent) 20 01, 03
Laboratory skills assessment worksheets (4) and in-class practical theory test (30min) (1000 words)30 01, 04, 05
Laboratory reports (2) and ethical assessment (1500 words equivalent)Includes 2 reports, 2 worksheets, clay embryo model and ethics debate40 01, 02, 03, 05
Journal club presentations (500 words equivalent)A 10 minute powerpoint/oral group presentation of a recent genetics research paper.10 02