Dr Kaye N.

Dr Kaye N. Truscott

Senior Research Fellow

College of Science, Health and Engineering

School of Molecular Sciences

Department of Biochemistry and Genetics

Room 418, LIMS1, Melbourne (Bundoora)

Research centres

La Trobe Institute for Molecular Science

Qualifications

BSc, DipEd, PhD (La Trobe University)

Role

Academic

Membership of professional associations

Australian Society for Biochemistry and Molecular Biology

Area of study

Biochemistry and Molecular Biology

Brief profile

For her doctorate, Kaye studied molecular chaperone assisted protein folding in thermophilic bacteria under the supervision of Prof. Robert Scopes. In 1999, shortly after obtaining her PhD, she was awarded an Alexander von Humboldt Research Fellowship to examine the protein import machinery in mitochondria of yeast and was hosted by Prof. Nikolaus Pfanner (Institute for Biochemistry and Molecular Biology, Freiburg, Germany). In 2001 Kaye was promoted to group leader within the Pfanner lab where she continued her studies on the molecular mechanisms of mitochondrial protein translocation. In 2004 she was awarded a QEII Fellowship by the Australian Research Council to study protein degradation by AAA+ proteases in bacteria and mitochondria. In 2009 Kaye was awarded an ARC Future Fellowship to extend her research in the area of mitochondrial protein homeostasis focusing on the role of AAA+ proteases in protein quality control and the mitochondrial unfolded protein response. Kaye is currently a Senior Research Fellow and is examining mitochondrial protein biogenesis pathways and protein homeostasis networks in relation to human health and disease. Kaye contributes to undergraduate lecturing and also post-graduate training through the supervision of practical classes and higher degree research students.

Research interests

Mitochondria biogenesis and disease

- Respiratory complex II assembly, Mitochondrial processing & quality control proteases

Neurodegenerative diseases

- Neurodegenerative diseases associated with mitochondrial protein dysfunction

Teaching units

BCH3BMA - Proteostasis

BIO5PRC - Practical Biotechnology (Subject & practical co-ordinator) 

Recent publications

Tan JL, Ward L, Truscott KN and Dougan DA (2016) The N-end rule adaptor protein ClpS from Plasmodium falciparum exhibits broad substrate specificity, FEBS Lett., 590: 3397-406.

Bezawork-Geleta A, Brodie EJ, Dougan DA and Truscott KN (2015) LON is the master protease that protects against protein aggregation in human mitochondria through direct degradation of misfolded proteins, Sci Rep., 5:17397, doi:10.1038/srep17397.

Micevski D, Zammit JE, Truscott KN and Dougan DA (2015) Anti-adaptors use distinct modes of binding to inhibit the RssB-dependent turnover of RpoS (σ(S)) by ClpXP, Front Mol Biosci., doi: 10.3389/fmolb.2015.00015.

Bezawork-Geleta A, Saiyed T, Dougan DA and Truscott KN (2014) Mitochondrial matrix proteostasis is linked to hereditary paraganglioma: LON-mediated turnover of the human flavinylation factor SDH5 is regulated by its interaction with SDHA, FASEB J., 28(4): 1794-804.

Nagpal J, Tan JL, Truscott KN, Heras B and Dougan DA (2013) Control of protein function through regulated protein degradation: biotechnological and biomedical applications, J Mol Microbiol Biotechnol., 23(4-5): 335-44.

Voos W, Ward L and Truscott KN (2013) The role of AAA+ proteases in mitochondrial protein biogenesis, homeostasis and activity control, Subcell Biochem., 66: 223-63.

Lowth BR, Kirstein-Miles J, Saiyed T, Brötz-Oesterhelt H, Morimoto RI, Truscott KN and Dougan DA (2012) Substrate recognition and processing by a Walker B mutant of the human mitochondrial AAA+ protein CLPX, J Struct Biol., 179(2): 193-201.

Böttinger L, Gornicka A, Czerwik T, Bragoszewski P, Loniewska-Lwowska A, Schulze-Specking A, Truscott KN, Guiard B, Milenkovic D and Chacinska A (2012) In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins, Mol Biol Cell, 23(20): 3957-69.

Dougan DA, Micevski D and Truscott KN (2012) The N-end rule pathway: From recognition by N-recognins, to destruction by AAA+ proteases, BBA Mol Cell Res., 1823(1): 83-91.

Gebert N, Gebert M, Oeljeklaus S, von der Malsburg K, Stroud DA, Kulawiak B, Wirth C, Zahedi RP, Dolezal P, Wiese S, Simon O, Schulze-Specking A, Truscott KN, Sickmann A, Rehling P, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N and Wiedemann N (2011) Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane, Mol Cell, 44(5): 811-18.

Truscott KN, Bezawork-Geleta A and Dougan DA (2011) Unfolded protein responses in bacteria and mitochondria: a central role for the ClpXP machine, IUBMB Life, 63(11): 955-63.