Staff profile

Dr Peter Lock

LIMS Microscopy and Flow Cytometry Manager, Senior Research Fellow

Faculty of Science, Technology and Engineering

School of Molecular Sciences
Department of Biochemistry
La Trobe Institute for Molecular Science

Physical Sciences 4, Melbourne (Bundoora)

 

Qualifications

PhD

Area of study

Biochemistry and Molecular Biology

Brief profile

 

Peter completed a B.Sc with First Class Honours in Biochemistry at La Trobe University in 1984. His PhD studies at the Ludwig Institute for Cancer Research (1987-91) examined the transcriptional and translational regulation of the emerging Src-family of proto-oncogenes. As a post doc at the Walter and Eliza Hall Institute of Medical Research (1991-94), Peter worked on the molecular basis of high affinity binding of GM-CSF, a hematopoietic growth factor, to its cell surface receptor. Peter then moved to the European Molecular Biology Laboratory in Germany for a 2nd post doc (1994-97), where he developed a novel tecnology to identify Src kinase substrates. This led to his discovery of the Tks5/Fish signalling protein at EMBL and two additional substrates, DokR and ARAP3, after his return to Australia (Ludwig Institute 1997-2000).

Peter has led his own research labs at the University of Melbourne (2001-09) and La Trobe University (2009-11) examining the biological functions of Src kinase substrates in biology and cancer. Over the past 5 years research has focused on the regulation of cytoskeletal structures known as invadopodia found on tumour cells which can degrade extracellular matrix components and may facilitate tumour invasion. This research has benefited extensively from the power of confocal microscopy to visualise the localisation and dynamics of invadopodia components. In a new role in 2012, Peter will manage and promote the La Trobe Institute for Molecular Science (LIMS) confocal microscopy and flow cytometry facilities.

Recent publications

 

22.King, J.A., Corcoran, N.M., D’Abaco, G.M., Straffon, A.F., Smith, C.M., Poon, C.L. Buchert, M., I, S., Hall, N.E., Lock, P., and Hovens, C.M. (2006). Eve-3: a liver enriched suppressor of Ras/MAPK signalling. J. Hepatol. 44, 758-67 (IMPACT FACTOR 2007     6.642, 8 citations). ERA ranking A  

23. Chan, J., Clements, W. Field, J., Nasa, Z., Lock P., Yap, F., Toh, B-H., and Alderuccio, F. (2006). Transplantation of bone marrow genetically engineered to express proinsulin II protects against autoimmune insulitis in NOD mice. J. Gene Med, 8, 1281-90. (FIVE YEAR IMPACT FACTOR 3.659,     12 citations) ERA ranking A  

24. Lock, P.,* I, S.T.T., Straffon, A.F., Schieb, H., Hovens, C.M. and Stylli, S.S. (2006). Spred-2 steady state levels are regulated by phosphorylation and Cbl-mediated ubiquitination. Biochem. Biophys. Res. Commun. 351, 1018-23. *corresponding author. (FIVE YEAR IMPACT FACTOR 2.802, 1 citation)   ERA ranking B  

25. Stylli, S.S., Kaye A.H., and Lock P.* (2008). Invadopodia: At the cutting edge of tumour invasion. J Clin Neurosci. 15, 725-37. *corresponding author. (FIVE YEAR IMPACT FACTOR 0.877, 46 citations). ERA ranking C  

26. Stylli, S.S. I, S.T.T., Verhagen, A.M., Xu S., Pass, I., Courtneidge, S.A., and Lock, P.* (2009). Nck adaptor proteins link Tks5 to invadopodia actin regulation and ECM degradation. J Cell Sci 122, 2727-40. *corresponding author. (FIVE YEAR IF 6.8, 10 citations)   ERA ranking A  

27. Stylli, S.S*., I, S.T.T., Kaye, A.H., and Lock, P.* (2012). Prognostic significance of Tks5 expression in gliomas. Journal of Clinical Neuroscience 19, 436 - 442. (FIVE YEAR IMPACT FACTOR 0.877). ERA ranking C. *these authors contributed equally.  This article was evaluated and recommended (category 6) by the Faculty of 1000, placing it in their library of the top 2% of articles in biology and medicine.

Older publications

 

1. Fincher, G.B., Lock, PA., Morgan, M.M., Lingelbach, K., Wettenhall, R.E.H., Mercer, J.B., Brandt, A., and Thomsen, K.K. (1986). Primary structure of the (1-3)(1-4)-beta-D-glucan-4-glucanohydrolase from barely aleurone. Proc. Natl. Acad. Sci. (USA). 83, 2081-85. (FIVE YEAR IMPACT FACTOR 10.369,   100 citations, in top 1% of cited articles in Plant/Animal Science)  

2. Albiston, A.L., Lock, P., Burger, H.G., and Krozowski, Z.S. (1990). Cloning and characterization of the rat alpha-inhibin gene. Mol. Cell. Endocrinol. 68, 121-8. (FIVE YEAR IMPACT FACTOR 3.037,   17 citations)  

3. Lock, P., Stanley, E., Holtzman, D.A., and Dunn, A.R. (1990). Functional analysis and nucleotide sequence of the promoter region of the murine hck gene. Mol. Cell. Biol. 10, 4603-11. (FIVE YEAR IMPACT FACTOR 6.825,   18 citations)  

4. Stanley, E., Ralph, S., McEwen, S., Boulet, I., Holtzman, D.A., Lock, P., and Dunn, A.R. (1991). Alternatively spliced murine lyn mRNAs encode distinct proteins. Mol. Cell. Biol. 11, 3399-406. (FIVE YEAR IMPACT FACTOR 6.825,    58 citations)  

5. Lock, P., Ralph, S., Stanley, E., Boulet, I., Ramsay, R., and Dunn, A.R. (1991). Two isoforms of murine hck, generated by utilization of alternative translational initiation codons, exhibit different patterns of subcellular localization. Mol. Cell. Biol. 11, 4363-70. (FIVE YEAR IMPACT FACTOR    6.825, 95 citations)  

6. Boulet, I., Ralph, S., Stanley, E., Lock, P., Dunn, A.R., Green, S.P., and Phillips,. W.A. (1992). Lipopolysaccharide- and interferon-gamma-induced expression of hck and lyn tyrosine kinases in murine bone marrow-derived macrophages. Oncogene 7, 703-10. (FIVE YEAR IMPACT FACTOR   6.371, 90 citations)  

7. Owczareck, C.M., Layton, M.J., Metcalf, D., Lock, P., Wilson, T.A., Gough, N.M., Nicola, N.A. (1993). Inter-species chimeras of leukemia inhibitory factor define a major human receptor-binding determinant. EMBO J. 12, 3487-95. (FIVE YEAR IMPACT FACTOR   9.275, 42 citations)  

8. Lock, P., Metcalf, D., and Nicola, N.A. (1994). Histidine-367 of the human common beta chain of the receptor is critical for high-affinity binding of human granulocyte-macrophage colony-stimulating factor. Proc. Natl. Acad. Sci. (USA). 91, 252-6. (FIVE YEAR IMPACT FACTOR 10.369, 43 citations). ERA ranking A*  

9. Layton, M.J., Lock, P., Metcalf, D., and Nicola, N.A. (1994). Cross-species receptor binding characteristics of human and mouse leukemia inhibitory factor suggest a complex binding interaction. J. Biol. Chem. 269, 17048-55. (FIVE YEAR IMPACT FACTOR 5.676,     32 citations) ERA ranking A*  

10. Layton, M.J., Owczarek, C.M., Metcalf, D., Lock, P.A., Wilson, T. A., Gough, N. M., Hilton, D. J., Nicola, N. A. (1994). Complex binding of leukemia inhibitory factor to its membrane-expressed and soluble receptors. Proc. Soc. Exp. Biol. Med. 206, 295-298. (FIVE YEAR IMPACT FACTOR 2.715, 7 citations).  

11. Metcalf, D., Willson, T., Rossner, M., and Lock, P. (1995). Receptor insertion into factor-dependent murine cell lines to develop specific bioassays for murine G-CSF and M-CSF and human GM-CSF. Growth Factors 11, 145-52. (FIVE YEAR IMPACT FACTOR     3.939) (15 citations)  

12. Lock, P., Fumagalli, S., Polakis, P., McCormick, F., and Courtneidge, S.A. (1996). The Human p62 cDNA encodes Sam68 and not the RasGAP-associated p62 protein. Cell 84, 23-4. (FIVE YEAR IMPACT FACTOR     28.779, 69 citations)  

13. Lock, P., Abram, C. L., Gibson, T., and Courtneidge, S.A. (1998). A new method for isolating tyrosine kinase substrates used to identify FISH, an SH3 and PX domain-containing protein, and Src substrate. EMBO J. 17, 4346-4357. (FIVE YEAR IMPACT FACTOR    9.275, 79 citations)  

14. *Lock, P, Casagranda, F., and Dunn, A.R. (1999). Independent SH2-binding sites mediate interaction of Dok-related protein with RasGTPase activating protein and Nck. J. Biol. Chem. 274, 22775-22784. *corresponding author. (FIVE YEAR IMPACT FACTOR 6.676,   42 citations)  

15. Lawrenson, I.D., Wimmer-Kleikamp, S.H., Lock, P., Schoenwaelder, S.M, Down, M., Reed, C., Boyd, A.W., Alewood, P.F., and Lackmann, M. (2002). Ephrin-A5 induces rounding, blebbing and de-adhesion of EphA3 expressing 293T and melanoma cells by CrkII and Rho-mediated signaling. J Cell Sci. 115,1059-72. (FIVE YEAR IMPACT FACTOR 6.779,     72 citations) ERA RANKING A      

16. Verhagen, A.M. and Lock, P. (2002). Revealing the intricacies of Cancer. Genome Biology 3, reports 4015.1-4015.5. (IMPACT FACTOR 2007    6.589)  

17. Gugasyan, R., Quilici, C., I, S.T.T, Grail, D., Verhagen, A.M., Roberts, A., Kitamura, T., Dunn, A.R. and Lock, P*. (2002). Dok-Related Protein Negatively Regulates T Cell Development via its RasGTPase-activating Protein and Nck Docking Sites. J Cell Biol. 158,115-125. *corresponding author. (FIVE YEAR IMPACT FACTOR   10.257, 22 citations)  

18. Abud, H.E., Lock, P. and Heath, J.K. (2004). Efficient Gene Transfer Into the Epithelial Cell Layer of Embryonic Mouse Intestine Using Low-Voltage Electroporation. Gastroenterology 126, 1779-1787. (FIVE YEAR IMPACT FACTOR 12.424,  7 citations)  

19. Grumont, R, Lock, P., Mollinari, M., Shannon, F., Moore, A., and Gerondakis, S. (2004). The Mitogen-Induced Increase in T Cell Size Involves PKC and NFAT Activation of Rel/NF-kB-Dependent c-myc Expression. Immunity 21, 19-30. (IMPACT FACTOR 2005    19.266, 33 citations)  

20. I, S.T.T, Nie, Z, Stewart, A., Hall, N.E., Najdovska, M., He, H., Randazzo, P.A., and Lock, P*. (2004). ARAP3 is transiently tyrosine phosphorylated in cells attaching to fibronectin and inhibits cell spreading in a RhoGAP dependent manner. J. Cell Sci. 117, 6071-6084.*corresponding author. (FIVE YEAR IMPACT FACTOR 6.779, 20 citations) ERA RANKING A  

21. King, J.A., Straffon, A.F., D'Abaco, G.M., Poon, C.L., I, S.T., Smith, C.M., Buchert, M., Corcoran, N.M., Hall, N., Callus, B.A., Sarcevic, B., Martin, D., Lock, P., Hovens, C.M. (2005). Distinct requirements for the Sprouty domain for functional activity of Spred proteins. Biochem J. 388, 445-454. (FIVE YEAR IMPACT FACTOR 4.074    11 citations) ERA RANKING A