Staff profile

Professor Graham D Lamb

NHMRC Senior Principal Fellow, Professor

Faculty of Science, Technology and Engineering
School of Life Sciences
Department of Zoology

Biological Sciences Building 1, Room 312, Melbourne (Bundoora)

Qualifications

MSc, PhD, UMELB

Membership of professional associations

Journal of Physiology, Journal of Muscle Research & Cell Motility, American Journal of Physiology Cell Physiology, Member of NH&MRC Research Fellowship Committee

Area of study

Biochemistry and Molecular Biology
Physiology
Zoology

Research interests

Muscle cell physiology

- Excitation-contraction coupling in health, exercise and disease

Teaching units

  • Excitable Tissues
  • Neuromuscular Disorders

 

Recent publications

  •  Dutka TL & Lamb GD (2004). Effect of carnosine on excitation-contraction coupling in mechanically-skinned fibres of rat skeletal muscle. Journal of Muscle Research & Cell Motility 25, 203-13.
  • Nielsen OB, Ortenblad N, Lamb GD & Stephenson DG (2004). Excitability of the T-tubular system in rat skeletal muscle: roles of K+ and Na+ gradients and Na+-K+ pump activity. Journal of Physiology 557, 133-46.
  • Murphy RM, Stephenson DG & Lamb GD (2004). Effect of creatine on contractile force and sensitivity in mechanically skinned single fibers from rat skeletal muscle. American Journal of Physiology Cell Physiology 287, C1589-95.
  • Pedersen TH, Nielsen OB, Lamb GD & Stephenson DG (2004). Intracellular acidosis enhances the excitability of working muscle. Science 305, 1144-1147.
  • Dutka TL & Lamb GD (2004). Effect of low cytoplasmic [ATP] on excitation-contraction coupling in fast-twitch muscle fibres of the rat. Journal of Physiology 560, 451-68.
  • Laver DR, O'Neill ER & Lamb GD (2004). Luminal Ca2+-regulated Mg2+inhibition of skeletal RyRs reconstituted as isolated channels or coupled clusters. Journal of General Physiology 124, 741-58.
  • Dulhunty AF, Cengia L, Young J, Pace SM, Harvey PJ, Lamb GD, Chan YN, Wimmer N, Toth I & Casarotto MG (2005). Functional implications of modifying RyR-activating peptides for membrane permeability. British Journal of Pharmacology 144, 743-54.
  • Lamb GD (2005). Rippling muscle disease may be caused by "silent action" potentials in the tubular system of skeletal muscle fibers. Muscle & Nerve 31, 652-8.
  • Verburg E, Murphy RM, Stephenson DG & Lamb GD (2005). Disruption of excitation-contraction coupling and titin by endogenous Ca2+-activated proteases in toad muscle fibres. Journal of Physiology 564, 775-90.
  • Dutka TL, Cole L & Lamb GD (2005). Calcium phosphate precipitation in the sarcoplasmic reticulum reduces action potential-mediated Ca2+release in mammalian skeletal muscle. American Journal of Physiology Cell Physiology 289, C1502-12.
  • Murphy RM, Snow RJ & Lamb GD (2006). mu-Calpain and calpain-3 are not autolyzed with exhaustive exercise in humans. American Journal of Physiology Cell Physiology 290, C116-22.
  • Verburg E, Dutka TL & Lamb GD (2006).  Long-lasting muscle fatigue: partial disruption of excitation-contraction coupling by the elevated cytosolic [Ca2+] during contractions. American Journal of Physiology Cell Physiology 290, C1199-1208.
  • Yang Z, Ikemoto N, Lamb GD & Steele DS (2006). The RyR2 central domain peptide DPc10 lowers the threshold for spontaneous Ca2+release in permeabilized cardiomyocytes. Cardiovascular Research 70, 475-485.
  • Trinh HH & Lamb GD (2006). Matching of sarcoplasmic reticulum and contractile properties in rat fast- and slow-twitch muscle fibres. Clinical and Experimental Pharmacology and Physiology 33, 591-600.
  • Murphy RM, Verburg E & Lamb GD (2006). Ca2+activation of diffusible and bound pools of mu-calpain in rat skeletal muscle. Journal of Physiology 576, 595-612.
  • Dutka TL & Lamb GD (2007a). Transverse tubular system depolarization reduces tetanic force in rat skeletal muscle fibers by impairing action potential repriming.  American Journal of Physiology Cell Physiology 292(6):C2112-21.
  • Dutka TL & Lamb GD (2007b). Na+-K+ pumps in the transverse tubular system of skeletal muscle fibers preferentially use ATP from glycolysis. American Journal of Physiology Cell Physiology 293(3):C967-77.
  • Murphy RM, Goodman CA, McKenna MJ, Bennie J, Leikis M & Lamb GD (2007). Calpain-3 is autolyzed and hence activated in human skeletal muscle 24 h following a single bout of eccentric exercise. Journal of Applied Physiology 103(3):926-31.
  • Laver DR, Honen BN, Lamb GD & Ikemoto N. (2008). A domain peptide of the cardiac ryanodine receptor regulates channel sensitivity to luminal Ca(2+) via cytoplasmic Ca(2+) sites. European Biophysical Journal 37, 455-67
  • Dutka TL, Murphy RM, Stephenson DG & Lamb GD. (2008). Chloride conductance in the transverse tubular system of rat skeletal muscle fibres: importance in excitation-contraction coupling and fatigue. Journal of Physiology 586: 875–887.
  • Allen DG, Lamb GD & Westerblad H. (2008a). Skeletal muscle fatigue; cellular mechanisms. Physiological Reviews 88, 287-332.
  • Allen DG, Lamb GD & Westerblad H. (2008b). Impaired calcium release during fatigue. Journal of Applied Physiology 104, 296-305.
  • Murphy RM, Dutka TL & Lamb GD. (2008). Hydroxyl radical and glutathione interactions alter calcium sensitivity and maximum force of the contractile apparatus in rat skeletal muscle fibres. Journal of Physiology 586, 2203-2216.
  • Mollica JP, Oakhill JS, Lamb GD, Murphy RM. (2009). Are genuine changes in protein expression being overlooked? Reassessing Western blotting. Anal Biochem. 386(2):270-5.
  • Murphy RM, Lamb GD. (2009). Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch. J Biol Chem. 284: 7811-9.
  • Murphy RM, Mollica JP, Lamb GD. (2009). Plasma membrane removal in rat skeletal muscle fibers reveals caveolin-3 hot-spots at the necks of transverse tubules. Exp Cell Res. 315: 1015-28.
  • Murphy RM, Larkins NT, Mollica JP, Beard NA, Lamb GD. (2009). Calsequestrin content and SERCA determine normal and maximal Ca2+ storage levels in sarcoplasmic reticulum of fast- and slow-twitch fibres of rat. J Physiol. 587: 443-60.
  • Dutka TL, Mollica JP, Lamb GD. (2011). Differential effects of peroxynitrite on contractile protein properties in fast- and slow-twitch skeletal muscle fibers of rat. Journal of Applied Physiology (in press).
  •  Dutka TL, Mollica JP, Posterino GS, Lamb GD. (2011). Modulation of contractile apparatus Ca2+-sensivitivity and disruption of excitation-contraction coupling by S-nitrosoglutathione in rat muscle fibres. Journal of Physiology (in press).
  •  Lamb GD, Westerblad H. (2011). Acute effects of reactive oxygen and nitrogen species on the contractile function of skeletal muscle. Journal of Physiology (in press).
  •  Murphy RM, Mollica JP, Beard NA, Knollmann BC, Lamb GD. (2011). Quantification of calsequestrin 2 (CSQ2) in sheep cardiac muscle and Ca2+-binding protein changes in CSQ2 knockout mice. American Journal of Physiology Heart & Circulation 300: H595-604.