Staff profile

Dr Daniel Tillett

Senior Lecturer

Faculty of Science, Technology and Engineering

School of Pharmacy and Applied Science
Department of Pharmacy

Applied Science 1, Room 2.12B, Bendigo

 

Qualifications

BSc Hons, PhD UNSW.

Area of study

Biotechnology

Research interests

Bioinformatics

- Molecular biology method development

Genomic and DNA sequencing

- Automated DNA sequencing reagents

Molecular virology

- Bacteriophages

Teaching units

 Microbiology

 Biotechnology

Recent publications

Chiu, J., P. E. March, R. Lee & D. Tillett (2004). Site-directed, Ligase Independent Mutagenesis (SLIM): A single-tube methodology approaching 100% efficiency in 4 hours. Nucleics Acids Research 32: e174.  

Neilan, B.A & D. Tillett (2002) Enzyme-free cloning of PCR products and fusion protein expression. Methods in Molecular Biology 192: 125-32.  

Tillett, D., D. P. Parker & B. A. Neilan (2001). Detection of toxigenicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus Microcystis: comparison of toxicities with 16S rRNA and phycocyanin operon (Phycocyanin Intergenic Spacer) phylogenies. Applied and Environmental Microbiology 67: 2810-8  

Tillett, D., E. Dittmann, M. Erhard, H. von Doehren, T. Boerner & B. A. Neilan (2000). Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC7806: an integrated peptide-polyketide synthetase system. Chemistry and Biology 7: 753-64  

Burns, B. P., D. Tillett, S. L. Hazell, G. L. Mendz T. Kolesnikow & B. A. Neilan (2000). The Helicobacter pylori pyrB gene encoding aspartate caramoyltransferase is essential for bacterial survival. Archives in Biochemistry and Biophysics 380: 78-84.  

Tillett, D. & B. A. Neilan (2000). Xanthogenate nucleic acid isolation from cultured and environmental Cyanobacteria. Journal of Phycology 36: 251-258.  

Tillett, D., B. P. Burns & B. A. Neilan (2000). Optimized rapid amplification of cDNA ends (RACE) for mapping bacterial mRNA transcripts. BioTechniques 28: 448-456.  

Tillett, D. & B. A. Neilan (1999). Enzyme-free cloning. Nucleic Acids Research 27: e26.  

Tillett, D. & B. A. Neilan (1999). Simple n-butanol purification of dye terminator sequencing reactions. BioTechniques 26: 606-610.  

Tillett, D. & B. A. Neilan (1999). An improved method for the purification of large DNA fragments from agarose gels using Wizard Plus SV columns. Analytical Biochemistry 269: 218-219.