Staff profile

Dr Chee-Kai Chan

Lecturer

Faculty of Science, Technology and Engineering

School of Molecular Sciences
Department of Genetics

Melbourne (Bundoora)

 

Qualifications

BSc, MEd (National University of Singapore), PGDipAppSc ( Lincoln University, NZ), PhD (ANU)

Membership of professional Associations

Australian Society for Medical Research, Australasian Gene Therapy Society

Area of study

Genetics

Brief Profile

  • 2002-03: Assistant Professor (Nanyang Technological University, Singapore) 
  • 2000-01: Post Doctoral Fellow (Johns Hopkins School of Medicine, Baltimore, USA).

 

Research interests

Anti-cancer drugs

- Drug delivery using ultrasound and polymeric nanoparticles

Gene Therapy

- Enhancing Non viral Gene delivery of Artificial Chromosomes

Teaching Units

GEN31HMG - Human Molecular genetics MS31MCG - Human Genome & Genetic diseases BIO12GEN - Genetics, Human Biology & Evolution GEN21HMG - Human and Molecular genetics

Recent Publications

     1.    Stephen R Doyle, Chee Kai Chan and Warwick Grant (2011) Enhanced annealing of mismatched oligonucleotides using a  novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism. BMC Biotechnology, 11:83doi:10.1186/1472-6750-11-83

2.    Chee Kai Chan, Adam Hart, Anna Lister, Stephen Doyle (2011). Learning styles of 3rd year Genetics Students and their Affection and Perception of effectiveness of a range of Instructional Modes. Proceedings of the Australian Conference on Science and mathematics Education. 121.

3.    Michael Ugarenko, Chee-Kai Chan, Abraham Nudelman, Ada Rephaeli, Suzanne M Cutts, Don R Phillips (2009).  Development of pluronic micelle-encapsulated doxorubicin and formaldehyde-releasing prodrugs for localized anticancer chemotherapy. Oncology Research. 17(7): 283-99

4.    Duy Huynh, Xu-Ming Dai, Sayan Nandi, Sally Lightowler, Melanie Trivett, Chee-Kai Chan, Ivan Bertoncello, Robert G Ramsay, E Richard Stanley. (2009) Colony stimulating factor-1 dependence of paneth cell development in the mouse small intestine. Gastroenterology.  137(1): 136-44, 144.

5.    Stephen R Doyle, Chee Kai Chan (2008).  Mitochondrial gene therapy: an evaluation of strategies for the treatment of mitochondrial DNA disorders. Human Gene Therapy. Dec;19(12): 1335-48

6.    Stephen R Doyle, Chee Kai Chan (2007). Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells. Genetic Vaccines and Therapy. 5: 11

7.    Duy D. Huynh, E. Vincan, T. Mantamadiotis, D. Purcell, CK Chan R. Ramsay (2007) Oncogenic properties of HIV-Tat in colorectal cancer cells. Current HIV Research, 5(4): 403-9.

8.    Tania Blanksby & Chee Kai Chan (2006) Feedback assessment of Science report writing for first year Genetics students . UNISERVE Symposium, Assessment in  Science Teaching and Learning. 161.

9.    Loveland, A.N. Chee-Kai Chan, Edward J. Brignole and Wade Gibson (2005) Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity. J Virol 79 (20), 12961-12968

10. Duy K. Pham and Chee Kai Chan. (2005) Impact of Polymeric Surfaces on  Oligonucleotides Immobilization for Lab-On-A-Chip Applications, Trends in DNA Research (Nova Science Publishers) Edit: Corey Woods

11. Chan, C.K. et al. (2002) Cytomegalovirus assemblin (pUL80a): cleavage at internal site not essential for virus growth; proteinase absent from virions. J Virol 76 (17), 8667-8674

12. Chan, C.K. and Jans, D.A. (2002) Using nuclear targeting signals to enhance non-viral gene transfer. Immunol Cell Biol 80 (2), 119-130

13. Chan, C.K. and Jans, D.A. (2001) Enhancement of MSH receptor- and GAL4-mediated gene transfer by switching the nuclear import pathway. Gene Ther 8 (2), 166-171

Older Publications

1. CK Chan, T Senden, DA Jans (2000). Supramolecular structure and nuclear targeting efficiency determine the enhancement of transfection by NLS-containing polylysine. Gene Therapy Oct 7; (19):1690-1697.

 

2. CK Chan and DA Jans (1999). Synergy of importin alpha recognition and DNA binding by the yeast transcriptional activator GAL4. FEBS Letters, Nov 26;462(1-2):221-4.

 

3. S Hubner, HMS Smith, W Hu, CK Chan, HP Rihs, BM Paschal, NV Raikhel and DA Jans (1999). Plant importin a binds nuclear localization sequences with high affinity and can mediate nuclear import independent of importin b. Journal of Biological Chemistry , Aug 6;274(32):22610-7.

 

4. CK Chan and DA Jans (1999). Enhancement of polylysine-mediated transfection by nuclear localization sequences (NLSs): polylysine does not function as an NLS.Human Gene Therapy, Jul 1;10(10):1695-702 

 

5. CK Chan, S Hubner, W Hu and DA Jans (1998). Mutual exclusivity of DNA binding and nuclear localization signal recognition by the yeast transcription factor GAL4: implications for nonviral DNA delivery. Gene Therapy, Sep;5(9):1204-12.

 

6. DA Jans, CK Chan & S Hubner (1998). Nuclear localization signals and the regulation of their activity by phosphorylation: application in drug delivery to the nucleus. Medicinal Research Reviews. 18 (4) 189-223.

Research projects

 Gene delivery and gene therapy

Vector development and improvement of gene transfer techniques by enhancing DNA-nuclear delivery.  Enhancement of non-viral delivery of large DNA constructs for gene therapy using ultrasound.

Gene delivery to the mitochondria. Construct development and direct delivery of DNA to mitochondria using peptide conjugates of mitochondriotropic complexes. 

Gene delivery and gene modification of Spermatogonia Stem cells (with Dr Adam Hart)

Non viral gene delivery for induced Pluripotent stem cells ( with Dr Adam Hart)

Ancient Human Migration and Peopling of Borneo ( with Dr John Mitchell)