Dr Connie

Dr Connie Darmanin

Research Fellow

College of Science, Health and Engineering

School of Molecular Sciences

Department of Chemistry and Physics

LIMS2, lab 215, Melbourne (Bundoora)

Qualifications

PhD

Role

Research fellow

Area of study

Biochemistry and Molecular Biology
Biology
Physics

Research interests

X-ray science

- X-ray Free electron laser, nanocrystallography, single molecule imaging, LCP crystallisation, GPCRs

Recent publications

N. Klonis, R. Dilanyan, Eric Hanssen, C. Darmanin, V. Streltsov, S Deed,  H. Quiney, L. Tilley. Hematin-hematin self-association states involved in the formation and reactivity of the malaria parasite pigment, hemozoin. Biochemistry, 2010, 49, 6804-6811

C.E. Conn, C. Darmanin, S. Sagnella, X. Mulet, T.L. Greaves, J. Varghese, C.J. Drummond. Incorporation of Dopamine D2L receptor and bacteriorhodopsin within bicontinuous cubic lipid phases. 1. Relevance to in meso crystallization of integral MPs in monoolein systems. Soft Matter, 6, 2010, 4828-4837 DOI: 10.1039/c0sm00463d.  

 C.E. Conn, C. Darmanin, S. Sagnella, X. Mulet, T.L. Greaves, J. Varghese, C.J. Drummond. Incorporation of Dopamine D2L receptor and bacteriorhodopsin within bicontinuous cubic lipid phases. 2. Relevance to in meso crystallization of integral MPs in novel lipid systems. Soft Matter, 6, 2010, 4838-4846 DOI: 10.1039/c0sm00464b  

C.E. Conn, X. Mulet, M. Moghaddam, C. Darmanin, L.J. Waddington, S.M. Sagnella, N. Kirby, J.N. Varghese, C.J. Drummond. Enhanced uptake of an integral membrane protein, the dopamine D2L receptor, by cubic nanoparticles doped with Ni chelated EDTA amphiphiles. Soft Matter, 2011, 7, 567-578  DOI: 10.1039/c0sm00790k  

Conn, Charlotte E, Darmanin C., Mulet, X; Moghaddam, M, Varghese, J  ; Drummond, CJ  Enhanced uptake of Integral Membrane Proteins by cubic nanoparticles. Biophysical J 2010, 98, 88A-88A 

R.A Dilanian, C. Darmanin, J.N Varghese, S.W Wilkins, T. Oka, N. Yagi, H.M. Quiney, K.A. Nugent. A new approach for structure analysis of two-dimensional membrane protein crystals using X-ray powder diffraction data. Protein Science 2011, 20, p 457-464   (IF=2.861, ISI=1)

Charlotte E. Conn, C. Darmanin, Xavier Mulet, Sophie LeCann, Nigel Kirby, and Calum J. Drummond. High-throughput analysis of the structural evolution of the monoolein cubic phase in situ under crystallogenesis conditions. Soft Matter, 2012, 8 (7), 2310 – 2321 

C. Darmanin, Charlotte E. Conn, Janet Newman, Xavier Mulet, Shane A. Seabrook, Yi-Lynn Liang, Adrian Hawley, Nigel Kirby, Joseph N.  Varghese, Calum J. Drummond. High-throughput production and structural characterization of libraries of self-assembly lipidic cubic phase materials, ACS Combinatorial Science, 2012, 14, 247-252 http://dx.doi.org/10.1021/co2001718 

Charlotte E. Conn, C. Darmanin, Xavier Mulet, Adrian Hawley, Calum J. Drummond. Effect of lipid architecture on cubic phase susceptibility to crystallisation screens. Soft Matter, 2012, 8, 6884-6896 DOI: 10.1039/c2sm25705j 

Leonie van’t Hag, C. Darmanin, Tu Le, Stephen Mudie, Charlotte E. Conn, Calum J. Drummond. In Meso Crystallization: Compatibility of Different Bicontinuous Cubic Mesophase-Forming Lipids in Aqueous Solutions with the Cubic Crystallization Screen. Crystal Growth & Design 02/2014 14(4):1771 1781. http://pubs.acs.org/doi/full/10.1021/cg4018954 

Y-L. Liang, C. E. Conn, C. J. Drummond, and C. Darmanin. Uptake of the butyrate receptors, GPR41 and GPR43, in lipidic bicontinuous cubic phases suitable for in meso crystallization. Journal of Colloidal and Interface Science, Journal of Colloid And Interface Science  (2015), 441, pp. 78-84 DOI information: 10.1016/j.jcis.2014.11.006 

H. D. Coughlan, C. Darmanin, N. W. Phillips, F. Hofmann, J.N. Clark, R. J. Harder, D. J. Vine, and B. Abbey. Radiation damage in a micron sized protein crystal studied via Reciprocal Space Mapping and Bragg Coherent Diffractive Structural Dynamics, 2, 041704 (2015); doi: 10.1063/1.4919641

Research projects

Current research project interests are in structural studies of G protein coupled receptors, nanocrystallography and X-ray Free Electron Laser Science.