Next-Generation Sequencing

The Genomics Platform features many state-of-the-art next generation sequencing instruments.

Services

  • Library preparation*: using standard Illumina kits
  • Sample/Library QC*:
  • Library sequencing:
  • Grants: work quoting / estimates for grant applications

Instruments

MiSeq

Applications

  • Small to medium scale sequencing capacity (<8 Gb)
  • De novo genome sequencing of small to medium sized genomes
  • Exome sequencing
  • Transcriptome sequencing
  • Genotyping by sequencing (RADseq)Targeted re-sequencing (e.g. sequencing of disease-associated genes in a patient cohort).

Capabilities

Regular

  • 300 bp PE
  • 25 million reads per run.

NextSeq 500

Capabilities

High-output

  • 150 PE
  • 400 million reads per run.

Mid-output

  • 150 PE
  • 130 million reads per run.

HiSeq 1500

Applications

  • De novo genome sequencing (unknown genomes)
  • Targeted resequencing - multiplexed PCR amplicons > exomes > known genomes
  • Transcriptome (RNA) sequencing (identify and quantify global mRNA expression patterns
  • Global analysis of DNA-protein interactions using ChIP-seq
  • Identification and quantification of micro RNAs without prior genome annotation
  • Genotyping by sequencing (RADseq)
  • Epigenetic sequencing of:
    • bisulfite-converted DNA
    • methylation-sensitive restriction digest-enriched fragments
    • anti-methyl C-precipitated fragments
    • chromatin IP of methyltransferases containing aza-labelled DNA.

Capabilities

High-output

  • 125 bp PE
  • 250 million reads per lane.

Rapid run

  • 150 bp PE
  • 150 million reads per lane.

2200 TapeStation

Applications

  • RNA, DNA and protein sample QC

Bioruptor Standard UCD-200

Diagenode’s Bioruptor ® Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems.

User Manual

Engagement

If you wish to submit some samples to be sequenced using our facility, please complete a Quote / Project Checklist below. Our team will contact you to arrange a quote and a date when it can be performed.

Quote / Project Checklist

If you wish to have a consultation it can be arranged after completing the checklist.

To access any of the auxiliary instruments please email genomics@latrobe.edu.au.

Price guideline

Please note that prices are an estimate based on current Illumina prices and may be subject to change.

Sequencing pricing [PDF 387KB].