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Department of Biochemistry
School of Molecular Sciences Safe Work Procedure
PHENOL
Use
Use of liquid Phenol and mixtures in DNA extraction and acid hydrolysis
reactions.
Hazards
Class 6.1 — Toxic. Poison, combustible and toxic via ingestion,
inhalation and skin absorption.
Risk Control Measures
Only use Phenol after receiving safety training (Laboratory
Induction and Authorisation). Phenol is easily absorbed through the skin;
fatalities have been documented from skin absorption via a relatively
small surface area. Use gloves, made of rubber or neoprene. Safety
glasses, goggles and face shield should be worn. Wear a laboratory coat.
If a splash potential exists, wear a rubber or neoprene apron.
Engineering / Ventilation Controls
Phenol is easily absorbed through the skin. Avoid skin contact.
Large quantities must be used in a fume hood. Use splash shielding if
splash or spray is possible. Ensure access to an eyewash facility in
areas where phenol is used.
Storage Requirements
This chemical is easily absorbed through skin.
Store away from oxidizers such as chlorine, bromine and hypochlorite.
First Aid / Spill Control Procedures
Wash off immediately with copious amounts of cold water (at least 10
minutes). Phenol is absorbed readily through the skin so follow the cold
water washing with a thorough washing with warm water and soap.
Contaminated clothing should be removed as soon as possible and
thoroughly washed.
In case of contact with eyes, immediately flush eyes with copious
amounts of water for at least 15 minutes (eyewash).
Seek medical attention. Poison Information Centre (0 13 1126).
Wear protective clothing.
Small spill:
Do not attempt clean up if you feel unsure of your ability to do so or
if you perceive the risk to be greater than normal laboratory
operations. Use vermiculite or Polyzorb spill absorbent material to soak
up spill. Place in labelled waste container ready for disposal. Flood
area with water then cover with caustic soda ash to neutralise the
residual material.
Large Spill:
Notify others in the area of spill. Evacuate area. Call the Molecular Sciences General Office (9479 2160), or Zisis Pouros (9479 1294) for clean up.
Barricade area with tape to prevent entry until arrival of response
personnel. Provide assistance and information to spill clean up crew.
Waste
Contact the Molecular Sciences General Office (9479 2160) for current waste disposal
instructions.
Applicable Standards and Regulations
Occupational Health and Safety (Hazardous Substances) Regulations
1999
Victorian Dangerous Goods (Storage and Handling) Regulations 1989.
Australian Standard AS 2243.10 (Safety in laboratories - Storage of
chemicals).
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