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Department of Biochemistry
School of Molecular Sciences Safe Work Procedure
ACIDS
Use
Mixing various concentrations of aqueous acid solutions. Handling of
strong acids.
Hazards
Class 8 – Corrosives. Acids - nitric, sulphuric, hydrochloric,
acetic, phosphoric. Risk of burns to skin and eyes.
Risk Control Measures
Always add concentrated acid to water to dilute – never add water to
acid. Only use concentrated acids after receiving safety training
(Laboratory Induction and Authorisation). Avoid skin contact, serious
burns may result. Gloves made of nitrile, neoprene, or PVC can provide
effective skin protection. Wear safety glasses or chemical safety
goggles with face shield when using large quantities, or chemical safety
goggles when using small quantities. Wear rubber, neoprene, or PVC apron
when using large quantities and splash potential exists.
Engineering / Ventilation Controls
Use concentrated acids in fume hood. An eyewash facility must be
available and accessible when working with corrosive liquids.
Storage Requirements
Store mineral acids together, separate from oxidizing agents and
organic materials. Store acetic acid and other organic acids with the
combustible organic liquids.
First Aid / Spill Control Procedures
Skin exposure: Rinse affected skin with plenty of water while
removing contaminated clothing and shoes. Rinse for at least 15 minutes.
Seek medical attention.
Eye exposure:
Splashes may cause tissue destruction. Wash eyes for at least 15
minutes, lifting the upper and lower eyelids occasionally. Seek medical
attention immediately.
Small spills:
Do not attempt clean up if you feel unsure of your ability to do so or
if you perceive the risk to be greater than normal laboratory
operations. Cover spill with sodium bicarbonate. When reaction stops
pick up with damp sponge or paper towels.
Large Spills:
Notify others in the area of spill. Use Polyzorb or vermiculite to
absorb liquid and prevent spill from spreading. Handle waste as you
would the spilled acid. Evacuate area. Call the Molecular Sciences General Office (9479
2160), or Zisis Pouros (9479 1294) for clean up. Barricade area with
tape to prevent entry until arrival of response personnel. Provide
assistance and information to spill clean up crew.
Waste Decontamination:
Use sodium bicarbonate and water.
Contact the Molecular Sciences General Office (9479 2160) for current waste disposal
instructions.
Applicable Standards and Regulations
Occupational Health and Safety (Hazardous Substances) Regulations 1999
Australian Standard AS 2243.10 (Safety in laboratories - Storage of
chemicals)
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