Research support
Advice and support is available on experimental design, live and fixed sample imaging, multipoint time series imaging, advanced fluorescence microscopy techniques (for example FRAP, FLIP, FRET, FLIM, FCS), image analysis and data presentation.
Useful Resources
- Wouters, F. S., Verveer, P. J., and Bastiaens, P. I. (2001) Imaging biochemistry inside cells. Trends in Cell Biology 11, 203–211
- Jares-Erijman, E. A., and Jovin, T. M. (2003) FRET imaging. Nature Biotechnology 21, 1387–1395
- Jares-Erijman, E. A., and Jovin, T. M. (2006) Imaging molecular interactions in living cells by FRET microscopy. Current Opinion in Chemical Biology 10, 409–416
- Esposito, A., Wouters, F. S., Bonifacino, J. S., Dasso, M., Harford, J. B., Lippincott-Schwartz, J., and Yamada, K. M. (2004) Fluorescence lifetime imaging microscopy. In: Bonifacino, J. S., Dasso, M., Harford, J. B., Lippincott-Schwartz, J., and Yamada, K. M. (Eds.) Current Protocols in Cell Biology. Wiley, New York, NY
- Reits, E. A. J., and Neefjes, J. J. (2001) From fixed to FRAP: measuring protein mobility and activity in living cells. Nature Cell Biology 3, E145–EE47
- Lippincott-Schwartz, J., Altan-Bonnet, N., and Patterson, G. H. (2002) Photobleaching and photoactivation: following protein dynamics in living cells. Nature Biotechnology 20, 87–90
- Lippincott-Schwartz, J., Snapp, E., and Kenworthy, A. (2001) Studying protein dynamics in living cells. Nature Reviews. Molecular cell biology 2, 444–456
- Ishikawa-Ankerhold, H.C., Ankerhold, R., and Drummen, G.P. (2012) Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM. Molecules 17, 4047-4132.
- Wouters, F. S., and Esposito, A. (2008) Quantitative analysis of fluorescence lifetime imaging made easy. HFSP Journal 2, 7-11
- Tsien, R.Y. The green fluorescent protein. Annu. Rev. Biochem. 1998, 67, 509–544.
