Equipment

Applications:

• Fast multi-channel confocal fluorescence imaging of live and fixed specimens (seven UV and visible laser lines)
• Highly dynamic cellular imaging applications. Simultaneous excitation and detection of up to 10 fluorescent dyes with overlapping emission spectra is possible using the sensitive 32 channel GaAsp spectral detector array
• Fluorescence lifetime imaging (FLIM) with single photon detection efficiency and picosecond resolution (using the integrated PicoQuant FLIM instrumentation)
• FLIM-based FRET
• Standard FRET, FRAP, FLIP, FLAP
• photo-activation and photo-conversion experiments

Applications:

• 3 colour high-speed imaging in X, Y and Z axes of live cells expressing fluorescent molecules, suitable for 3D time series reconstructions
• Physiological studies requiring low phototoxicity and photobleaching (Achieved by gentle sample illumination via the Yokogawa spinning disk and ultra-sensitive Photometrics Evolve EMCCD cameras).
• Dual camera and filter configuration capable of simultaneous live imaging of GFP/DsRed or CFP/YFP combinations

Applications:

• Three colour confocal fluorescence imaging of live and fixed cells (6 visible laser lines)
• Applications requiring low phototoxicity and photobleaching are accommodated by high quantum efficiency avalanche photodiode (APD) detectors which can be used as an alternative to less sensitive PMT detectors
• Standard FRET, FRAP, FLIP, FLAP and photo-activation experiments
• Fluorescence Correlation Spectroscopy (FCS)

Applications:

• Three colour confocal point scanning fluorescence imaging of specimens on slides (6 visible laser lines)
• Precise spectral imaging of fluorescent proteins and dyes (Leica spectral prism and slit mechanism)
• Fluorescence recovery after photobleaching (FRAP)

Applications:

• Four channel widefield fluorescence imaging of live and fixed specimens
• Two camera configuration enables sensitive fluorescence imaging as well as high resolution colour imaging (e.g. H&E stained tissue sections)
• Wide range of objective lenses for low and high power imaging (2x to 100x)
• Automated multichannel imaging of cell dynamics with OKO stage-top incubator
• Bright field phase contrast imaging

Applications:

• Four colour widefield fluorescence imaging of fixed cells mounted on slides
• Bright field differential interference contrast (DIC) imaging

Applications:

• Three colour widefield fluorescence imaging of cells and tissues mounted on slides
• Bright field and differential interference contrast (DIC) imaging