Microscopy and Imaging

Microscopy and imaging

The LIMS Microscopy and Imaging Facility maintains four confocal and three widefield fluorescence microscopes with advanced capabilities for imaging of live and fixed biological samples.

Imaging applications handled by facility equipment include:

  • standard fluorescence imaging
  • confocal imaging (point scanning and spinning disk systems available)
  • Forster resonance energy transfer (FRET)
  • fluorescence lifetime imaging (FLIM)
  • FLIM-based FRET
  • fluorescence recovery after photobleaching (FRAP)
  • fluorescence loss in photobleaching (FLIP)
  • fluorescence localisation after photobleaching (FLAP)
  • photo-activation and photo-conversion
  • fluorescence correlation spectroscopy (FCS)
  • fluorescence spectral detection and unmixing
  • high-speed and long-term live cell imaging with full incubation (temperature, CO2 and humidity control)
  • Bright-field techniques include phase contrast, differential interference contrast (DIC) and high-resolution full colour imaging.
A dedicated workstation equipped with Imaris, ZEN, SymPhotime, ImageJ/Fiji and Metamorph software is available for 2D, 3D and 4D image data analysis and rendering.

 

Equipment

The LIMS Microscopy and Imaging Facility has a large range of instrumentation and services.

Bookings

Information about training, fees and how to book equipment in the LIMS Microscopy and Imaging Facility.

Research support

Support and resources for researchers using the LIMS Microscopy and Imaging Facility.

Contact

For more information about the LIMS Microscopy and Imaging Facility, please contact:

Dr Peter Lock
LIMS Microscopy Manager
T: +61 3 9479 1669
E: P.Lock@latrobe.edu.au

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