Histology
The LIMS Histology Facility offers a suite of precision instruments for producing high quality tissue sections on slides. Sections are suitable for histochemical or immunochemical staining techniques and light microscopy-based investigations (fluorescence and bright-field) of tissue histology, pathology and cytology.
Key capabilities of the facility include:
- embedding of tissues in paraffin or OCT compound
- automated specimen processing
- precise thin sectioning of fresh, cryostat-frozen and paraffin-embedded tissues.
Equipment
Leica TP1020 tissue processor
Applications:
- Automated fixation, dehydration and xylene impregnation of tissue specimens.
- Pre-processing of tissues prior to paraffin embedding.
Leica EG1150 tissue embedder
Applications:
- Embedding of tissue specimens in paraffin for subsequent microtome sectioning
- Used in conjunction with the Leica RM2235 microtome
Leica RM2235 microtome
Applications:
- Manual precision sectioning of paraffin embedded tissue (2- 40 µm range)
- Capable of sectioning relatively hard materials
Leica CM1850 cryostat microtome
Applications:
- Rapid specimen freezing
- Cryosectioning of frozen OCT-embedded tissues (2-60 µm range)
Leica VT1200 vibrating blade microtome
Applications:
- Precision sectioning of fresh tissue samples (2- 40 µm range)
- Particularly suited to sectioning of brain tissues
- Tissue sections suitable for physiology studies such as patch-clamping
Funding support
LIMS gratefully acknowledges the Clive and Vera Ramaciotti and Rebecca L Cooper Foundations for providing funds to purchase the Leica cryostat and vibrating blade microtomes respectively.
Contact
Dr Jacqueline Orian
T: +61 3 9479 1113
E: J.Orian@latrobe.edu.au
