Research
Selected publications
Teaching
Higher degree supervision
Back to staff member's homepage

RESEARCH/INTERESTS

Cellular and molecular mechanism of the catabolism of matrix proteoglycans by articular cartilage, ligament, tendon and joint capsule.

Molecular organization of proteoglycans within the extracellular matrix of articular cartilage and connective tissue of the synovial joint.

Regulation of proteoglycan catabolism by sulfated polysaccharides and amino sugars.

RECENT PUBLICATIONS

Book Chapters

Hascall, V.C., Sandy, J.D.& Handley, C.J. (1999). Regulation of Proteoglycan Metabolism in Articular Cartilage, Biology of the Synovial joints, Amsterdam: Harwood Academic Publishers, pp 101-120.

Journal Articles

Ilic, M, Carter, P, Tyndall, A, Dudhia, J & Handley, C Proteoglycans and catabolic products of proteoglycans present in ligament Biochemical Journal, 385 (2) 2005 381-388.

Handley, C., Ilic, M., Samiric, T., Large aggregating and small leucine-rich proteoglycans are degraded by different pathways and at different rates in tendon., European Journal of Biochemistry., 271( 17 ) 2004, 3612-3620.

Samiric, T., Ilic, M., Handley, C., Characterization of proteoglycans and their catabolic products in tendon and explant cultures of tendon., Matrix Biology, 23( 2 ) 2004, 73-140.

Vankemmelbeke, M N, Jones, G C, Fowles, C, Ilic, M, Handley, C, Day, A J, Knight, C, Mort, J & Buttle, D J, Selective inhibition of ADAMTS-1, -4 and-5 by catechin gallate esters, European Journal Biochemistry, 270 (11), 2003, 2394-2403.

Ilic, M, Martinac, B & Handley, C, Effects of long-term exposure to glucosamine and mannosamine on aggrecan degration in articular cartilage., Osteoarthritis and Cartilage, 11 (8), 2003, 613-622.

Munteanu, S.E, Ilic, M.Z., and Handley, C.J., 'Highly sulfated glycosaminoglycans inhibit aggrecanase degradation of aggrecan by bovine articular cartilage explant cultures', Matrix Biology, Vol 21, Issue 5, pp 429-440, 2002.

Handley, C.J., Winter, G.M., Ilic, M.Z., Ross, J.M., Poole, A.C. and Robinson C.H., 'Distribution of Newly Synthesised Aggrecan in explant cultures of bovine cartilage treated with retinoic acid', Matrix Biology, Vol 21, Issue 7, pp 579-92, 2002.

Handley, C.J., Mok, M.T. Ilic, M.Z., Adcocks C., Buttle, D.J. Cathepsin D cleaves aggrecan at unique sites within the interglobular domain and chondroitin sulfate attachment regions that are also cleaved when cartilage is maintained at acid PH. (2001) Matrix Biology. Vol: 20 (8) pp 543-53

Vankemmelbeke M.N., Holen I., Wilson A.G., Ilic, M.Z., Handley C.J. Expression and activity of ADAMTS-5 in synovium. (2001) Eur J Biochem. Vol: 268 (5) pp 1259-68

Campbell, M. A., Tester, A. M., Handley, C. J., Checkley, G. J., Chow, G. L., Cant, A. D., Winter, A. D. and Cain, W. E. (1996). Characterization of a large chondroitin sulphate proteoglycan present in bovine collateral ligament. Archives of Biochemistry and Biophysics, 329, 181-190.

Campbell, M. A., Winter, A. D., Ilic, M. Z. and Handley, C. J. (1996). Catabolism and loss of proteoglycans from cultures of bovine collateral ligaments. Archives of Biochemistry and Biophysics, 328, 64-72.

Karopoulos, C., Rowley, M. J., Ilic, M. Z. and Handley, C. J. (1996). Synovial fluid antibodies to aggrecan in rheumatoid arthritis and other articular diseases react with the G1 domain of native aggrecan. Arthritis and Rheumatism, 39, 1990-1997.

Yang, Z., de Veer, M. J., Gardiner, E. E., Devenish, C. J., Handley, C. J., Underwood, J. R. and Robinson, H. C. (1996). Rabbit polymorhonuclear neutrophils form 35S-labeled S-sulfo-calgranulin when incubated with inorganic [35S] sulfate. Journal of Biological Chemistry, 19802-19809.

Buttle, D. J., Fowles, A., Ilic, M. Z. & Handley, C. J. (1997). "Aggrecanase" activity is implicated in tumour necrosis factor mediated cartilage aggrecan breakdown but is not detected by an in vitro assay. Journal of Clinical Pathology - Clinical Molecular Pathology Edition, 50, 153-159.

Ilic, M.Z., Robinson, H.C., Handley, C.J. (1998). Characterization of aggrecan retained and lost from the extracellular matrix of articular cartilage. Journal of Biological Chemistry, 272, 17451-17458.

Winter, G.M., Poole, C.A., Ilic, M.Z., Robinson H.C. and Handley C.J. (1998) Identification of distinction metabolic pools of aggrecan and their relationship to type VI collagen in the chondrons of mature bovine articular cartilage explants. Connective Tissue Research, 37, 277-293.

Tester, A.M., Ilic, M.Z., Robinson, H.C & Handley, C.J. (1999). Metabolic processing of newly synthesized link protein in bovine articular cartilage explant cultures. Matrix Biology, 18, 65-74.

Vankemmelbeke, M.N., Ilic, M.Z., Handley, C.J., Knight, C.G., and Buttle, D.J. (1999). Co-incubation of bovine synovial or capsular tissue with cartilage generates a soluble "aggrecanase" activity. Biochemical and Biophysical Research Communications, 255, 686-691.

Winter, A.D., Campbell, M.A., Robinson, H.C., & Handley, C.J. (2000). Catabolism of newly synthesized decorin by explant cultures of bovine ligament. Matrix Biology, 19 129-138.

Ilic, M.Z., Vankemmelbeke, M.N., Holen, I., Buttle, D.J., Robinson, H.C., Handley, C.J. (2000). Bovine joint capsule and fibroblasts derived from joint capsule express aggrecanase activity. Matrix Biology, 19, 257-265.

Munteanu, S.E., Ilic, M.Z., Handley, C.J. (2000). Calcium pentosan polysulfate inhibits the catabolism of aggrecan in articular cartilage explant cultures. Arthritis and Rheumatism, 43, 2211 8.

Vankammelbeke, M.N., Holen, I., Wilson, A.G., Ilic, M.Z., Handley, C.J., Kelner, G.S., Clark, M., Liu, C., Maki, R.A., Burnett, D. and Buttle, D.J. (2001). Expression and activity of ADAMTS-5 in synovium. European Journal of Biochemistry, 268, 1259-1268.

Conference Publications

Lay, E, Yammouni, C M, Samiric, T, Ilic, M & Handley, C (ed/s), Effect of quercitin on aggrecanase activity in bovine articular cartilage explant cultures (Matrix Biology Society of Australia and New Zealand : Parkville, Victoria, 2005).

Martinac, B, Samiric, T, Ilic, M & Handley, C (ed/s), The effect of glucosamine on proteoglycan loss from explant cultures of ligament and tendon (Matrix Biology Society of Australia and New Zealand : Parkville, Victoria, 2005).

Samiric, T, Ilic, M & Handley, C (ed/s), Catabolism of Newly Synthesised Proteoglycans in Tendon and the Effects of Highly Sulfated Polysaccharides on this Process ( Matrix Biology Society of Australia and New Zealand : Parkville, Victoria , 2004 )

Ilic, M, Carter, P, Tyndall, A, ‡Dudhia, J & Handley, C (ed/s), Proteoglycans and catabolic products of proteoglycans present in ligament. ( Matrix Biology Society of Australia and New Zealand. : Parkville, Victoria. , 2004 )

Samiric, T, Ilic, M & Handley, C, Catabolism of Proteoglycans in Tendon, in Convenor: Dr. Amanda Fosang(ed/s), 27th Annual Meeting of the Matrix Biology Society of Australia and New Zealand (Matrix Biology Society of Australia and New Zealand: Melbourne, 2003) 29.

Samiric, T, Ilic, M & Handley, C, Characterisation of proteoglycans and their degradation products of bovine tendon, in Chair: Roberto Perris(ed/s), 3rd International Conference on Proteoglycans - The Harden Conferences (Medicina Viva: Parma, Italy, 2003) 12.

Samiric, T, Ilic, M & Handley, C, The kinetics and catabolic fate of newly synthesized proteoglycans in explant cultures of bovine tendon, in Chair: Roberto Perris(ed/s), 3rd International Conference on Proteoglycans - The Harden Conferences (Medicina Viva: Parma, Italy, 2003) 13.

Dooley, P, Howgate, M, Handley, C, Schuijers, J & Grills, B, Early, Soft tissue fracture callus contracts and relaxes in vitro., in Ego Seeman(ed/s), International Conference of Metabolic Bone Disease/Australian and New Zealand Bone and Mineral Society. (Australian and New Zealand Bone and Mineral Society: Australia, 2003) 116.

Handley, C.J., Munteanu, S., & Ilic M.Z. Suppression of aggrecanase mediated degradation of aggrecan by highly sulfated polysaccharides, 2002 Annual Meeting of Matrix Biology Society of Australia and New Zealand, Vol 26, p20, Wiseman's Ferry, Australia, 25-28 September 2002.

Ilic, M.Z., Martinac, B., and Handley C.J., 'Effects of long-term exposure to glucosamine and mannosamine on aggregan degradation in explant cultures of articular cartilage', Vol 26, p27, Matrix Biology Society of Australia and New zealand Annual Meeting, Wisemans Ferry Australia, 25-28 September 2002.

Munteanu, S.E., Ilic, M.Z., and Handley, C.J., 'Short-term exposure of explant cultures of articular cartilage to pentosan polysulfate results in long-term protection to aggrecan loss', Osteoarthritis Research International, Vol 10 supplement A, p 545, Sydney Australia, 25-28 September 2002.

Ilic, M.Z., Martinac, B., and Handley C.J., 'Effects of Glucosamine on the catabolism of aggregan in articular cartilage, Osteoarthritis Research International, Vol 10 supplement A, Sydney Australia, 25-28 september 2002.

Vankemmelbeke, M.N., Jones, C.C., Fowles, C., Ilic, M.Z., Handley, C.J., and Day, A.J., 'Inhibition of AdamTS-1, -4 and 5 by catecin, Vol 26, p19, Matrix Biology Society of Australia and New Zealand, Annual meeting, Wisemans Ferry Australia, 25-28 September, 2002

Handley, C.J., Vankemmelbeke M.N., Jones, G.C., Holen, I., Ilic, M.Z. Investigations into the proteolytic activity of recombinant human ADAMTS-5. Proteoglycans: Message in the Matri. Page 49

Handley, C.J., Munteanu, S. Ilic, M.Z. (2001). Attenuation of Aggrecanase-Mediated Degradation of Aggrecan by Over-Sulfated Polysaccharides. 'The many faces of osteoarthritis - an international conference. Page 51

Handley, C.J., (2001) Articular Cartilage, Structure, Function and its Potential for Repair. Institute of Bone and Joint Research Symposium. Page No: 7

TEACHING

General molecular physiology. Physiology and function of connective tissues.

HIGHER DEGREE SUPERVISION (current)

Doctor of Philosophy:

Shannon Munteanu "Regulation of cartilage catabolism by sulfonated polysaccharides" 1999 - 2003

Belinda Martinac "Effects of glucosamine and mannosamine on the catabolism of aggrecan in articular cartilage." 2000-

Alicia Tyndall "The role of relaxin and blood supply in skeletal metabolism". 2003 -