Science, Technology and Engineering

Dougan & Truscott Lab

Department of Biochemistry

Recent Research Publications

Kirstein J, Moliere N, Dougan DA & Turgay K (2009). Adapting the machine: Adaptor proteins for Hsp100/Clp and AAA+ proteases. Nature Reviews Microbiology 7, 589-599.

Ninnis RL, Spall SK, Talbo GH, Truscott KN & Dougan DA (2009). Natural substrates of the N-end rule pathway in Escherichia coli. EMBO Journal 28, 1732-1744.

Schuenemann VJ, Kralik SM, Albrecht R, Spall SK, Truscott KN, Dougan DA & Zeth K (2009). Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS. EMBO Reports 10, 508-514.

Erbse AH, Wagner JN, Truscott KN, Spall SK, Kirstein J, Zeth K, Turgay K, Mogk A, Bukau B. & Dougan DA (2008). Conserved residues in the N-domain of the AAA+ chaperone ClpA, regulate substrate recognition and unfolding FEBS Journal 275, 1400-1410.

Wagner K, Gebert N, Guiard B, Brandner K, Truscott KN, Wiedemann N, Pfanner N, Rehling P. (2008). The assembly pathway of the mitochondrial carrier translocase involves four preprotein translocases. Mol Cell Biol. 28, 4251-4260.

Kirstein J, Dougan DA, Gerth U, Hecker M, Turgay K (2007). The tyrosine kinase McsB is a regulated adaptor protein for ClpCP. EMBO J. 26, 2061-2070.

Kirstein J, Schlothauer T, DOUGAN DA, Lilie H, Tischendorf G, Mogk A, Bukau B, Turgay K. (2006) Adaptor protein controlled oligomerization activates the AAA+ protein Clp. EMBO J., 25, 1481-91.

Meinecke M, Wagner R, Kovermann P, Guiard B, Mick DU, Hutu DP, Voos W, TRUSCOTT KN, Chacinska A, Pfanner N & Rehling P. (2006) Tim50 Maintains the Permeability Barrier of the Mitochondrial Inner Membrane. Science 312, 1523-6

Erbse A, Schmidt R, Bornemann T, Schneider-Mergener J, Mogk A, Zahn R, DOUGAN DA, Bukau B. (2006) ClpS is an essential component of the N-end rule pathway in Escherichia coli. Nature 439, 753-6.

Chacinska A, Lind M, Frazier AE, Dudek J, Meisinger C, Geissler A, Sickmann A, Meyer HE, TRUSCOTT KN, Guiard B, Pfanner N & Rehling P. (2005) Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120, 817-29.

Brandner K, Rehling P & TRUSCOTT KN (2005) The carboxy-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocase. J. Biol. Chem., 280, 6215-21.

Weibezahn J, Tessarz P, Schlieker C, Zahn R, Maglica Z, Lee S, Zentgraf H, Weber-Ban EU, DOUGAN DA, Tsai FT, Mogk A, Bukau B. (2004) Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB. Cell. 119, 653-65.

Chacinska A, Pfannschmidt S, Wiedemann N, Kozjak V, Sanjuan Szklarz LK, Schulze-Specking A, TRUSCOTT KN, Guiard B, Meisinger C & Pfanner N. (2004) Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins. EMBO J. 23, 3735-46.

Frazier AE, Dudek J, Guiard B, Voos W, Li Y, Lind M, Meisinger C, Geissler A, Sickmann A, Meyer HE, Bilanchone V, Cumsky MG, TRUSCOTT KN, Pfanner N & Rehling P. (2004) Pam16 has an essential role in the mitochondrial protein import motor. Nat. Struct. Mol. Biol. 11, 226-33.

DOUGAN DA, Weber-Ban E, Bukau B. (2003) Targeted delivery of an ssrA-tagged substrate by the adaptor protein SspB to its cognate AAA+ protein ClpX. Mol Cell. 12, 373-80.

Schlothauer T, Mogk A, DOUGAN DA, Bukau B, Turgay K. (2003) MecA, an adaptor protein necessary for ClpC chaperone activity. Proc. Natl. Acad. Sci. U S A. 100, 2306-11.

TRUSCOTT KN, Voos W, Frazier AE, Lind M, Li Y, Geissler A, Dudek J, Muller H, Sickmann A, Meyer HE, Meisinger C, Guiard B, Rehling P & Pfanner N. (2003) A J-protein is an essential subunit of the presequence translocase-associated protein import motor of mitochondria. J. Cell. Biol. 163, 707-13.

Rehling P, Model K, Brandner K, Kovermann P, Sickmann A, Meyer HE, Kuhlbrandt W, Wagner R, TRUSCOTT KN, Pfanner N. (2003) Protein insertion into the mitochondrial inner membrane by a twin-pore translocase. Science. 299, 1747-51.

Zeth K, Ravelli RB, Paal K, Cusack S, Bukau B, DOUGAN DA. (2002) Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA. Nat. Struct. Biol. 9, 906-11.

Geissler A, Chacinska A, TRUSCOTT KN, Wiedemann N, Brandner K, Sickmann A, Meyer HE, Meisinger C, Pfanner N, Rehling P. (2002) The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell. 111, 507-18.

TRUSCOTT KN, Wiedemann N, Rehling P, Muller H, Meisinger C, Pfanner N, Guiard B. (2002) Mitochondrial import of the ADP/ATP carrier: the essential TIM complex of the intermembrane space is required for precursor release from the TOM complex. Mol. Cell. Biol. 22, 7780-9.

Johnston AJ, Hoogenraad J, DOUGAN DA, TRUSCOTT KN, Yano M, Mori M, Hoogenraad NJ, Ryan MT. (2002) Insertion and assembly of human tom7 into the preprotein translocase complex of the outer mitochondrial membrane. J. Biol. Chem. 277, 42197-204.

DOUGAN DA, Reid BG, Horwich AL, Bukau B. (2002) ClpS, a substrate modulator of the ClpAP machine. Mol Cell. 9, 673-83.

Kovermann P, TRUSCOTT KN, Guiard B, Rehling P, Sepuri NB, Muller H, Jensen RE, Wagner R, Pfanner N. (2002) Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel. Mol Cell. 9, 363-73.

TRUSCOTT KN, Kovermann P, Geissler A, Merlin A, Meijer M, Driessen AJ, Rassow J, Pfanner N, Wagner R. (2002) A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23. Nat. Struct. Biol. 8, 1074-82.

For other publications by this author:

This will open a new browser window and connect you in to the NCBI PubMed database. Please close the new window to come back to this page.