Facilities - Microscope Imaging & Analysis Facility
Department of Biochemistry
Specifications for the TCS SP2 confocal laser scanning microscope
Inverted microscope (Leica DM IRE2 HC Fluo TCS 1-B)
- motorized coarse and fine focusing
- motorised prism light direction
- incident fluorescence light module with motorized filter turret
- 13 (blue excitation BP 450 – 490; LP emission LP 515) typical for FITC
- N2.1 (green excitation BP 515 – 560; LP emission LP 590) typical for TRITC
- Galvanometer driven z-stage
Objectives
- HC PL FLUOTAR 10x/0.30 Ph1
- HCX PL APO 40x/1.25 Oil Ph3 CS
- HCX PL APO 63x/1.32 Oil Ph3 CS
- HCX PL APO 63x/1.20 W Corr/0.17 CS
- PL APO 100x/1.40 Oil Ph3 CS
DIC and Phase contrast optics
TCS SP 2 Confocal Scan Head with Spectral Detection System
- Continuously adjustable bandwidth and center wavelength.
- Three channels for confocal fluorescence or reflected light.
- Variable, computer controlled pinhole diameter
- Line frequency up to 2000 lines/sec.
- Frame rates 3 fps at 512x512; 25 fps at 512x32 pixels
- Scan resolution up to 4096x4096 pixels
Laser sources
- Blue (Ar 458nm/5mW; 476nm/5mW; 488nm/20mW; 514nm/20mW
- Green (HeNe 543nm/1.2mW)
- Red (HeNe 633nm/10mW)
Software
- 3D Software License LCS
- Physiology Software License LCS
- Multicolor Software License LCS
- Macro Developer Software License LCS
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