Staff profile

Dr Mark Hulett

Senior Lecturer, Research Director La Trobe Institute for Molecular Science

Faculty of Science, Technology and Engineering
School of Molecular Sciences
Department of Biochemistry
La Trobe Institute for Molecular Science

Physical Sciences 4 room 216/216a, Melbourne (Bundoora)


BSc Hons, PhD UMELB.

Membership of professional associations

Australian Society for Medical Research, Australian Society for Biochemistry and Molecular Biology, American Society for Biochemistry and Molecular Biology, Australasian Society for Immunology

Area of study

Biochemistry and Molecular Biology

Brief profile

Dr Hulett completed his undergraduate studies at The University of Mebourne majoring in Biochemistry and Cell Biology and his PhD studies in the field of Molecular Immunology at the Austin Research Institute. In 1998 Mark was awarded an NHMRC postdoctoral fellowship and joined the John Curtin School of Medical Research (JCSMR) in Canberra where he performed pioneering research by cloning a key enzyme in cell invasion, heparanase. Dr Hulett was awarded a Viertel Senior Medical Research Fellowship in 2002, and established the Cancer and Molecular Immunology laboratory at JCSMR to study cell invasion during inflammation, tumour metastasis and angiogenesis. In 2008, Dr Hulett moved his lab to La Trobe University to continue his research in inflammatory disease and tumour progression. Dr Hulett was president of the Australia Society for Medical Research in 2008. He has published over 60 research articles and won numerous awards, including a Young Tall Poppy Award and the Howard Florey Medal.

Research interests include:

- Molecular basis of cell invasion in tumour metastisis, angiogenesis and inflammation, to develop novel strategies to combat cancer and inflammatory disease.

Teaching units

Course coordinator:

SBMS Bachelor of Biomedical Science

SHMES Bachelor of Biomedical Science with Honours



BCH3BMB Biochemistry and Molecular Biology 3B

MED3MSA Advanced Topics in Medical Science

MED2BMS Introduction to Biomedical Science



Hexima Ltd

Recent publications

Lay, F.T., Mills, G.D., Poon, I.K., Cowieson, N.P., Kirby, N., Baxter, A.A., van der Weerden, N.L., Dogovski, C., Perugini, M.A., Anderson, M.A., Kvansakul*, M. and HULETT*, M.D. 2012. Dimerization of the plant defensin NaD1 enhances its antifungal activity. J Biol Chem. 2012 Apr 17. [Epub ahead of print]

Lay, F.T., Veneer P.K., HULETT*, M.D. and  Kvansakul* M. 2012.  Recombinant expression and purification of the tomato defensin TPP3 and its preliminary X-ray crystallographic analysis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68:314-6.

Ishii, H., HULETT, M.D., Li, J.M., Santiago, F.S., Parish, C.R. and Khachigian, L.M. 2012. Yin Yang-1 inhibits tumor cell growth and inhibits p21WAF1/Cip1 complex formation with cdk4 and cyclin D1. Int J Oncol. 2012 40:1575-80.

Lay, F.T., Mills, G.D., HULETT*, M.D. and Kvansakul*,  M. 2011 Crystallization and preliminary X-ray crystallographic analysis of the plant defensin NaD1. Acta Crystallogr Sect F Struct  Biol Cryst Commun. 68: 85-8.

Poon, I.K., Patel, K.K., Davis, D.S., Parish*, C.R. and HULETT*, M.D. 2011. Histidine-rich glycoprotein: the Swiss Army knife of mammalian plasma. Blood. 117:2093-101.

Poon, I.K.A., HULETT*, M.D. and Parish*. C.R. 2010. Molecular mechanisms of late apoptotic/necrotic cell clearance. Cell Death Differ. 17: 381-97.

Poon, I.K.H, HULETT*, M.D. and Parish*, C.R. 2010.  Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcRI on phagocytyes. Blood. 115: 2473-82.

Poon IK, Parish* CR, and HULETT*, M.D. 2010. Histidine-rich glycoprotein functions cooperatively with cell surface heparan sulfate on phagocytes to promote necrotic cell uptake.  J Leukoc Biol. 88: 559-69.

Poon IK, Yee DY, Jones AL, Wood RJ, Davis DS, Freeman C, Parish* CR and HULETT*, M.D. 2010. Histidine-rich glycoprotein binds heparanase and regulates its enzymatic activity and cell surface interactions. Int J Biochem Cell Biol. 42:1507-16.

Poon, I.K.H, Olsoon, A.K., HULETT*, M.D. and Parish*, C.R.  2009.  Regulation of histidine-rich glycoprotein (HRG) function via plasmin-mediated proteolytic cleavage.  J. Biochem.  424: 27-37.

Kavallaris, M., Meachem, S.J., HULETT, M.D., West, C.M., Pitt, R.E., Chesters, J.J., Laffan, W.S., Boreham, P.R., Khachigian, L.M. 2008 Perceptions in health and medical research careers: the Australian Society for Medical Research Workforce Survey. Med J Aust. 188:520-524.

Quah, B., Barlow, V.P., McPhun, V., Matthaei, K.I., HULETT, M.D. and Parish, C.R. 2008. Bystander B Cells Rapidly Acquire Antigen Receptors from Activated B Cells by Membrane Transfer: a Novel Mechanism for Enhancing Specific Antigen Presentation. Proc. Natl Acad. Sci. U. S. A. 2008 105:4259-4264.

Wood, R. and HULETT M.D. 2007. Cell surface expressed cation-independent mannose-6 phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose-6 phosphate to promote extracellular matrix degradation. J. Biol. Chem.  283:4165-4176

de Mestre, A.M., Staykova, M., Hornby, J., Willenborg, D. and HULETT M.D. 2007. The expression of the heparan sulphate degrading enzyme heparanase is induced in infiltrating CD4+ T cells in experimental autoimmune encephalomyelitis and regulated at the level of transcription by early growth response gene 1 . J. Leuk. Biol. 82:1289-300.

de Mestre, A.M., Soe-Htwe, T., Sutcliffe, E.L., Rao, S., Pagler, E.B., Hornby, J.R. and HULETT, M.D. 2007. Regulation of mouse Heparanase gene expression in T lymphocytes and tumour cells. Immunol. Cell Biol. 85:205-214.


Older publications


Forbes, E., HULETT, M.D., Ahens, R., Smart, V., Matthaei, K.I., Brandt, E., Rothenberg, M.E., Tang, M., Foster, P.S. and Hogan, S.P. 2006. ICAM-1 dependent pathways regulate colonic eosinophilic inflammation. J. Leuk. Biol. 80:330-41.

Abby, J.L., HULETT M.D. and O’Neill H. 2006. Cell surface expression of a peptide encoded by the un-rearranged TCR-Vbeta 8.2 gene. Mol. Immunol. 43:1408-1417.

Jones, A.L., Poon, I., HULETT, M.D. and Parish, C. R. 2005. Histidine-rich glycoprotein specifically binds to necrotic cells, via its amino-terminal domain, and facilitates necrotic cell phagocytosis. J. Biol. Chem. 280:35733-35741.

Jones, A.L., HULETT, M.D. and Parish, C. R. 2005. Histidine-rich glycoprotein: A novel adaptor protein in plasma that modulates the immune, vascular and coagulation systems. Immunol Cell Biol. 83:106-18.

de Mestre, A.M, Rao, S., Hornby, J.R., Soe Htwe, Khachigian, L.M. and HULETT M.D. 2005. EGR1 regulates heparanase gene transcription in tumour cells. J. Biol. Chem. 280:35136-35147.

Jones, A.L., HULETT, M.D., Altin, J.G., Hogg, P. and Parish C.R. 2004. Plasminogen is tethered with high affinity to the cell surface by the plasma protein, histidine-rich glycoprotein. J Biol. Chem. 279: 38267-38276.

Jones, A.L., HULETT, M.D. and Parish C.R. 2004. Histidine-rich glycoprotein binds to cell surface heparan sulfate via its amino-terminal domain following Zn2+ chelation. J. Biol. Chem. 279:30114-30122.

de Mestre, A.M., Khachigian L.M., Santiago, F.S., Staykova, M.A. and HULETT M.D. 2003. Regulation of inducible heparanase gene transcription in activated T cells by early growth response 1. J. Biol. Chem. 278:50377-50385.

Mattes, J., HULETT, M.D., Xie, W., Hogan, S., Rothenberg, M.E., Foster, P. and Parish C.R. 2003. Immunotherapy of cytotoxic T cell resistant tumors by T helper 2 cells: an eotaxin and STAT6 dependent process. J. Exp. Med. 197: 387-393.

Mackay, G.A., HULETT, M.D., Cook, J.P.D., Trist, H.M., Henry, A.J., Beavil, A.J., Beavil, R.L., Sutton, B.J., Hogarth, P.M. and Gould H.J. 2002. Mutagenesis within human FcRI differentially affects human and murine IgE. J. Immunol., 168, 1787-1795

Parish, C. R., C. Freeman and M.D. HULETT. 2001. Heparanase: a key enzyme involved in cell invasion. Biochimica et Biophysica Acta - Reviews in Cancer 1471: M99-M108.

HULETT, M.D., Pagler, E. and Hornby J.R. 2001. Cloning and characterisation of a mouse homologue of the human hematopoietic cell-specific four-transmembrane gene HTm4. Immunol Cell Biol., 79, 345-349.

HULETT, M.D., E. Pagler, J. Hornby, P.M. Hogarth, H.J. Eyre, E. Baker, J. Crawford, G.R. Sutherland and Parish, C.R. 2001. Isolation, tissue distribution, and chromosomal localisation of a novel testis-specific human four-transmembrane gene related to CD20 and FcRI. Biochem. Biophys. Res. Commun., 280, 374-379.

HULETT, M.D., J. Hornby, S. Ohms, J. Zeugg, C. Freeman, J. Greedy and C.R. Parish. 2000. Identification of active site residues of the pro-metastatic endoglycosidase heparanase. Biochemistry, 39, 15659-15667.

Rigby, L.J., H. Trist, H., V.C. Epa, J. Snider, M.D. HULETT and P.M. Hogarth. 2000. Monoclonal antibodies and synthetic peptides define the active site of FcRI and a potential receptor antagonist. Allergy, 55, 609-619.

Rigby, L.J., V.C. Epa, G.A. Mackay, M.D. HULETT, B.J. Sutton, H.J. Gould and P.M. Hogarth. 2000. Domain one of the high affinity Fc epsilon receptor, FcRI, regulates binding to IgE through its interface with domain two. J. Biol. Chem., 275, 9664-9672.

HULETT, M.D. and C.R. Parish. 2000. Murine Histidine-rich Glycoprotein: Cloning, characterisation and cellular origin. Immunol. Cell Biol., 78, 280-287.

Baker, E., J. Crawford, G.R. Sutherland, C. Freeman, C.R. Parish, and M.D. HULETT. 1999. Human Hpa. Endogylcosidase heparanase. Map postion 4q21.3. Chromosome Research 7, 319.

HULETT, M.D., C. Freeman, B.J. Hamdorf, R.T. Baker, M.J. Harris, and C.R. Parish. 1999. Cloning of mammalian heparanase: A key enzyme in tumour invasion and metastasis. Nature Medicine 5:803-809.

Maxwell, K.F., M.S. Powell, M.D. HULETT, P.A. Barton, I.F.C. McKenzie, T.P. Garrett and P.M. Hogarth. 1999. Crystal structure of the human leukocyte Fc receptor FcRIIa. Nature Structural Biology 6:437-442.

HULETT, M.D. E. Witort, R. Brinkworth, I.F.C. McKenzie and P.M. Hogarth. 1999. Fine structure analysis of the interaction of FcRI with IgE. J. Biol. Chem. 274:13345-13352.

Wines, B. D., M.D. HULETT, G.P. Jamieson, H.M. Trist, J.M. Spratt, and P.M. Hogarth. 1999. Identification of residues in the first domain of Human FcR essential for interaction with IgA. J. Immunol. 162:2146-2153. 

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