Development of new methodologies to monitor the progression of cell death and disassembly of dying cells by flow cytometry

The use of annexin V and 7-aminoactinomycin D/propidium iodide stains to quantify cell death by flow cytometry has been considered the gold standard by most laboratories. However, this widely used method often makes the assumption that there are only three types of particles in a sample, that is viable, apoptotic and necrotic (membrane permeabilized) cells.

In order to study the progression of cell death in greater detail, in particular how apoptotic cells undergo fragmentation to generate apoptotic bodies, we aim to establish new flow cytometry-based methods to accurately measure the cell death process.